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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production

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Pereira Scarpa, Josiani de Cassia [1] ; Marques, Natalia Paganini [2] ; Monteiro, Diego Alves [1] ; Martins, Gisele Marta [1] ; de Paula, Ariela Veloso [3] ; Boscolo, Mauricio [1] ; da Silva, Roberto [1] ; Gomes, Eleni [1] ; Bocchini, Daniela Alonso [2]
Total Authors: 9
[1] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

Pycnoporus swsguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 degrees C. The highest endoglucanase, exoglucanase, beta-glucosidase, xylanase, beta-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g(-1), respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g(-1) for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L-1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 degrees C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained. (AU)

FAPESP's process: 11/19554-0 - Prospecting of thermophilic filamentous fungi producers of cellulases and application of the enzymes in sugarcane bagasse saccharification
Grantee:Daniela Alonso Bocchini
Support Opportunities: Regular Research Grants