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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach

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Author(s):
Grella, Tatiane Caroline [1, 2] ; Soares-Lima, Hellen Maria [3] ; Malaspina, Osmar [2] ; Ferreira Nocelli, Roberta Cornelio [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Ctr Ciencias Agr, Dept Ciencias Nat Matemat & Educ, Campus Araras, Rodovia Anhanguera SP-330, Km 174, BR-13600970 Araras, SP - Brazil
[2] Univ Estadual Paulista, Dept Biol, Ctr Estudos Insetos Socials, Ave 24-A, 1-515, BR-13506900 Rio Claro, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Biol, Ctr Ciencias & Tecnol Sustentabilidade, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Chemosphere; v. 236, DEC 2019.
Web of Science Citations: 2
Abstract

During foraging, bees are exposed to sublethal doses of insecticides, which can cause morphological changes to various organs, such as the midgut, Malpighian tubules, and mushroon body. Thus, the purpose of this study was to establish a scoring system to evaluate these alterations based on the damage caused and its reversibility. Therefore, a grade 1 score indicates a minimal and easily reversible lesion, increased apocrine secretion, increased cell elimination into the lumen, and a larger quantity of spherocrystals; grade 2 was assigned to moderate and typically reversible injuries, such as changes in the brush border, vacuolation/loss of cytoplasmic material, presence/height of the brush border, and cell swelling; and grade 3 was assigned to serious and irreversible, loss of cell nests of regenerative cells, pyknosis, and loss of contact between Kenyon cells. In addition, frequency values were assigned since the alterations can occur at different frequencies according to the insecticide and the bees exposed; the frequency ranges from 0 to 6, with 0 representing the absence of an alteration and 6 representing a high frequency occurrence. Based on the analyses, we conclude that each change causes morphological damage, which may or may not be irreversible and could affect the health of the colony. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
Grantee:Osmar Malaspina
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 13/09555-5 - Synergistic effects between Nosema sp. and insecticide imidacloprid on honeybees africanized Apis mellifera
Grantee:Hellen Maria Soares Lima
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/12488-0 - Risk assessment of pesticides on bees and its impact on the pollination process
Grantee:Roberta Cornélio Ferreira Nocelli
Support type: Regular Research Grants