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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dietary hemin promotes colonic preneoplastic lesions and DNA damage but not tumor development in a medium-term model of colon carcinogenesis in rats

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Author(s):
de Moura, Nelci A. [1] ; Caetano, Brunno F. R. [1] ; Bidinotto, Lucas T. [2, 3] ; Rodrigues, Maria A. M. [4] ; Barbisan, Luis F. [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, UNESP, Botucatu, SP - Brazil
[2] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos, SP - Brazil
[3] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil
[4] Sao Paulo State Univ, Sch Med, Dept Pathol, UNESP, Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 846, OCT 2019.
Web of Science Citations: 0
Abstract

Red and processed meat consumption has been strongly related to increase the risk of colorectal cancer (CRC), although its impact is largely unknown. Hemin, an iron-containing porphyrin, is acknowledged as a putative factor of red and processed meat pro-carcinogenic effects. The aim of this study was to investigate the effects of high dietary hemin on the promotion/progression stages of 1,2-dimethylhydrazine (1,2-DMH)-induced colon carcinogenesis. Twenty-four Wistar male rats were given four subcutaneous 1,2-DMH injections and received either balanced diet or balanced diet supplemented with hemin 0.5 mmol/kg for 23 weeks. Colon specimens were analyzed for aberrant crypt foci (ACF) and tumor development. Dietary hemin significantly increased ACF number and fecal water cytotoxicity/genotoxicity in Caco-2 cells when compared to 1,2-DMH control group. However, tumor incidence, multiplicity and cell proliferation did not differ between 1,2-DMH + hemin and 1,2-DMH control group. Gene expression analysis of 91 target-genes revealed that only three genes (Figf, Pik3r5 and Tgfbr2) were down-regulated in the tumors from hemin-fed rats compared to those from 1,2-DMH control group. Therefore, the findings of this study show that high hemin intake promotes mainly DNA damage and ACF development and but does not change the number nor incidence of colon tumors induced by 1,2-DMH in male rats. (AU)

FAPESP's process: 11/23699-4 - Effects of symbiotic and indole-3-carbinol intake on colon carcinogenesis process in Wistar rats fed a diet containing heme.
Grantee:Nelci Antunes de Moura
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/12631-2 - Effects of symbiotic and indole 3-carbinol on the gene expression involved in colon carcinogenesis in rats fed a diet containing heme
Grantee:Luís Fernando Barbisan
Support Opportunities: Regular Research Grants
FAPESP's process: 13/08033-5 - Fecal water genotoxicity and development of aberrant crypt foci in male Wistar rats fed heme, indole-3-carbinol and/or symbiotic formulation
Grantee:Brunno Felipe Ramos Caetano
Support Opportunities: Scholarships in Brazil - Scientific Initiation