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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteomic Analysis of Lipid Rafts from RBL-2H3 Mast Cells

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Freitas Filho, Edismauro Garcia [1] ; Marin Jaca, Luiz Augusto [1] ; Baeza, Lilian Cristiane [2] ; de Almeida Soares, Celia Maria [2] ; Borges, Clayton Luiz [2] ; Oliver, Constance [1] ; Jamur, Maria Celia [1]
Total Authors: 7
[1] Univ Sao Paulo, Dept Cell & Mol Biol & Pathogen Bioagents, Ribeirao Preto Med Sch, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Fed Goias, Biol Sci Inst, Mol Biol Lab, Samambaia Campus 2, ICB2, Room 206, BR-74690900 Goiania, Go - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Lipid rafts are highly ordered membrane microdomains enriched in cholesterol, glycosphingolipids, and certain proteins. They are involved in the regulation of cellular processes in diverse cell types, including mast cells (MCs). The MC lipid raft protein composition was assessed using qualitative mass spectrometric characterization of the proteome from detergent-resistant membrane fractions from RBL-2H3 MCs. Using two different post-isolation treatment methods, a total of 949 lipid raft associated proteins were identified. The majority of these MC lipid raft proteins had already been described in the RaftProtV2 database and are among highest cited/experimentally validated lipid raft proteins. Additionally, more than half of the identified proteins had lipid modifications and/or transmembrane domains. Classification of identified proteins into functional categories showed that the proteins were associated with cellular membrane compartments, and with some biological and molecular functions, such as regulation, localization, binding, catalytic activity, and response to stimulus. Furthermore, functional enrichment analysis demonstrated an intimate involvement of identified proteins with various aspects of MC biological processes, especially those related to regulated secretion, organization/stabilization of macromolecules complexes, and signal transduction. This study represents the first comprehensive proteomic profile of MC lipid rafts and provides additional information to elucidate immunoregulatory functions coordinated by raft proteins in MCs. (AU)

FAPESP's process: 13/12861-0 - The role of mast cell specific gangliosides in modulating mediator release
Grantee:Edismauro Garcia Freitas Filho
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/17671-8 - Mast cell specific gangliosides modulate mediator release
Grantee:Maria Célia Jamur
Support Opportunities: Regular Research Grants
FAPESP's process: 17/14645-4 - The involvement of RACK1 in the release of mast cell mediators.
Grantee:Maria Célia Jamur
Support Opportunities: Regular Research Grants
FAPESP's process: 15/16673-0 - The role of the scaffold protein RACK1 in mast cell activation
Grantee:Edismauro Garcia Freitas Filho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/18618-1 - Interaction between mast cells and endothelial cells during in vitro angiogenesis
Grantee:Constance Oliver
Support Opportunities: Regular Research Grants