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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of tryptoquialanines A and C produced by Penicillium digitatum in oranges: Are we safe?

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Araujo, Eder de Vilhena [1] ; Vendramini, Pedro Henrique [1] ; Costa, Jonas Henrique [1] ; Eberlin, Marcos Nogueira [1] ; Montagner, Cassiana Carolina [1] ; Fill, Taicia Pacheco [1]
Total Authors: 6
[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Food Chemistry; v. 301, DEC 15 2019.
Web of Science Citations: 0

A method was developed and validated for determination of tryptoquialanines A and C in orange samples on epicarp (exterior peel), mesocarp (white peel), and endocarp (fruit juice) based on QuEChERS extraction and LC-MS/MS analysis. The method showed an excellent linearity over a range of 5-400 mu g kg(-1), with r(2) >= 0.998. The limits of detection (LOD) and quantification (LOQ) were 5 and 10 mu g kg(-1), respectively. Recoveries showed values between 57 and 101%, with RSD <= 12%. Analysis of infected oranges showed diffusion of the alkaloids between the orange layers after 4 days post infection in concentrations > LOQ. Mycotoxin diffusion to healthy oranges after direct contact with infected oranges for 48 h, showed alkaloid concentrations >= 10 mu g kg(-1) on epicarp layer. The developed method can be easily applied for quality control in routine analysis of orange fruit due to the high risk that these tremorgenic alkaloids represent to human health. (AU)

FAPESP's process: 18/03670-0 - Prospecting for anticancer secondary metabolites produced by phytopathogenic fungi
Grantee:Jaqueline Moraes Bazioli
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/10384-6 - Biosynthetic studies involving bottom-up approaches of polyketides produced by Penicillium species
Grantee:Taicia Pacheco Fill
Support Opportunities: Regular Research Grants
FAPESP's process: 17/24462-4 - Understanding the virulence strategies of Penicillium digitatum against the host citrus
Grantee:Taicia Pacheco Fill
Support Opportunities: Regular Research Grants