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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities

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Author(s):
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Raphael A. de Jesus [1] ; Vilma M. J. Prado [2] ; Vanderson S. Pinto [3] ; Valdenizia R. Silva [4] ; Luciano S. Santos [5] ; Paulo C. L. Nogueira [6] ; Sandro Navickiene [7] ; Edenir R. Pereira-Filho [8] ; Arie F. Blank [9] ; Daniel P. Bezerra [10] ; Milena B. P. Soares ; Claudia Seidl [12] ; Carmen L. Cardoso [13] ; Valéria R. S. Moraes [14]
Total Authors: 14
Affiliation:
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[1] Universidade Federal de Sergipe. Departamento de Química - Brasil
[2] Universidade Federal de Sergipe. Departamento de Química - Brasil
[3] Universidade Federal de Sergipe. Departamento de Engenharia Agronômica - Brasil
[4] Fundação Oswaldo Cruz. Instituto Gonçalo Moniz - Brasil
[5] Fundação Oswaldo Cruz. Instituto Gonçalo Moniz - Brasil
[6] Universidade Federal de Sergipe. Departamento de Química - Brasil
[7] Universidade Federal de Sergipe. Departamento de Química - Brasil
[8] Universidade Federal de São Carlos. Departamento de Química - Brasil
[9] Universidade Federal de Sergipe. Departamento de Engenharia Agronômica - Brasil
[10] Fundação Oswaldo Cruz. Instituto Gonçalo Moniz - Brasil
[12] Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Química - Brasil
[13] Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Química - Brasil
[14] Universidade Federal de Sergipe. Departamento de Química - Brasil
Total Affiliations: 14
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 30, n. 5, p. 978-987, 2019-04-08.
Abstract

The methodology developed in this study through the acquisition of fingerprint chromatograms by liquid chromatography-diode array detector (LC-DAD), aided by principal component analysis, made it possible to assess, in a rational way, the chemical differences between six accessions of Lippia alba and to verify the influence of the method of extraction, the seasonality and the individuality of each accession on chemical composition of its extracts. Among all extracts analyzed against cancer cell lines, eight of them showed to be more promising against a human leukemia cell line (HL-60), displaying cell growth inhibition percentage ranging between 40.0 and 52.0%. In the inhibitory activity assays against acetylcholinesterase enzyme, all extracts showed weak inhibitory effect, with highlight only for six of them, which displayed inhibition ranging between 27.0 and 32.0%. (AU)

FAPESP's process: 13/01710-1 - Enzyme ligand: new models of screening
Grantee:Quezia Bezerra Cass
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/11640-3 - LC-MS / 2D methods for screening ligands for evaluating enzyme inhibitory activity and identification of active compounds in crude extracts
Grantee:Cláudia Seidl
Support Opportunities: Scholarships in Brazil - Post-Doctoral