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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Amyloidogenic model peptides as catalysts for stereoselective aldol reactions

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Author(s):
Pelin, Juliane N. B. D. [1] ; Gerbelli, Barbara B. [1] ; Soares, Bruna M. [1] ; Aguilar, Andrea M. [2] ; Alves, Wendel A. [1]
Total Authors: 5
Affiliation:
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil
[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CATALYSIS SCIENCE & TECHNOLOGY; v. 9, n. 16, p. 4304-4313, AUG 21 2019.
Web of Science Citations: 0
Abstract

In this work, the self-assembly in an aqueous medium of two compounds derived from amyloid-like structures, {[}RF](4) and P{[}RF](4) (where: R = arginine; F = phenylalanine; P = proline), was studied by using spectroscopy, microscopy, and scattering techniques. The peptides presented a similar critical aggregation concentration (cac) considering the same medium conditions (similar to 0.35 mmol L-1 at 25 degrees C). The stability of the systems was analyzed by zeta potential measurements, which showed that under acidic and neutral conditions the colloidal particles remained stable, and for basic solutions, strongly interacting colloids and supramolecular aggregates in solution were observed. CD data confirmed the changes in the conformation of the secondary structures for both compounds by changing the pH of the solution. By SAXS, the curves were fitted using a fractal structure factor, and we observed that the size of the monomers that compose the fractal aggregates of {[}RF](4) is twice as large as that of P{[}RF](4). These compounds showed potential as catalysts in aldol reactions, using cyclohexanone and p-nitrobenzaldehyde. In this case, under optimized reaction conditions, 78% conversion (ee up to 85%) was observed in {[}RF](4) solutions, while P{[}RF](4) had only 51% conversion (ee up to 85%). Our results show, for the first time, a dependence of the morphology on the stereoselective aldol reactions, whereas the folding arrangement of the chiral assemblies in the polypeptide chains plays an essential role in the access of the reactants to the active surface sites. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Lauro Tatsuo Kubota
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties
Grantee:Adalberto Fazzio
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/05888-3 - Biosensors based on amphiphilic peptides for detection and diagnosis of diseases
Grantee:Barbara Bianca Gerbelli
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/20446-9 - Study of auto-organization in solution of hierarchical structures of peptide in presence of metallic nanoparticles
Grantee:Juliane Nogueira Batista Dias Pelin
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)