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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Full-Thickness Intraoral Mucosa Barrier Models for In Vitro Drug-Permeation Studies Using Microneedles

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Serpe, Luciano [1] ; Muniz, Bruno Vilela [1] ; dos Santos, Cleiton Pita [1] ; da Silva, Camila Batista [1] ; Volpato, Maria Cristina [1] ; Groppo, Francisco Carlos [1] ; Vianna Lopez, Renata Fonseca [2] ; Franz-Montan, Michelle [1]
Total Authors: 8
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Pharmaceutical Sciences; v. 108, n. 5, p. 1756-1764, MAY 2019.
Web of Science Citations: 1

The use of permeation enhancers such as microneedles (MNs) to increase drug penetration across intraoral mucosa has increased in recent years. Permeation studies, commonly performed using vertical diffusion cells, are a well-established way to preview formulations and enhance their performance during the development stage. However, to our knowledge, the existing intraoral mucosa barrier models do not permit permeation using MN-pretreated mucosa due to their insufficient thickness. Therefore, the objective of this study was to develop a barrier model using thick palate tissues to perform in vitro permeation studies, with physical enhancement of the permeability of intraoral mucosa by pretreatment with MNs. The adapted Franz-type cells used in the permeation experiments were validated (cell dimensions and volume, sealing effectiveness, stirring and dissolution efficiency, temperature control, and establishment of uniaxial flux). Commercially available MNs were used in the palatal mucosa. Optical images of the mucosa were acquired to analyze the microperforations created. In vitro permeation studies were conducted with the MN-pretreated mucosa. This work presents a new in vitro method for the evaluation of MNs as permeation enhancers, with the aim of improving the absorption of drug formulations topically applied within the oral cavity. (c) 2019 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved (AU)

FAPESP's process: 12/06974-4 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics
Grantee:Michelle Franz Montan Braga Leite
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/02492-5 - In vitro study of the microneedles system appicability for topical anesthesia in swine oral tissues.
Grantee:Luciano Serpe
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/18113-0 - Development of methods to perform permeation studies of topical formulations for dentistry applications
Grantee:Francisco Carlos Groppo
Support Opportunities: Regular Research Grants