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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sperm-borne miR-216b modulates cell proliferation during early embryo development via K-RAS

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Rodrigues Alves, Maira Bianchi [1] ; de Arruda, Rubens Paes [1] ; De Bem, Tiago Henrique Camara [2] ; Florez-Rodriguez, Shirley Andrea [1] ; de Sa Filho, Manoel Francisco [1, 3] ; Belleannee, Clemence [4] ; Meirelles, Flavio Vieira [2] ; da Silveira, Juliano Coelho [2] ; Perecin, Felipe [2] ; Carvalho Celeghini, Eneiva Carla [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP - Brazil
[3] Alta Genet Brazil, Uberaba, MG - Brazil
[4] Univ Laval, Dept Obstet Gynecol & Reprod, Quebec City, PQ - Canada
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, JUL 17 2019.
Web of Science Citations: 0

Semen fertilizing potential is dependent upon the morphological, functional and molecular attributes of sperm. Sperm microRNAs (miRNAs) were recently shown to hold promise regarding their association with different fertility phenotypes. However, their role in fertility regulation remains to be determined. We postulated that sperm miRNAs might regulate early embryonic development. From this perspective, sperm quality and 380 sperm miRNAs were investigated in frozen-thawed semen from high (HF; 54.3 +/- 1.0% pregnancy rate) and low (LF; 41.5 +/- 2.3%) fertility bulls. Out of nine miRNAs that showed different levels in sperm cells, miR-216b was present at lower levels in HF sperm cells and zygotes. Among miR-216b target genes (K-RAS, BECN1 and JUN), K-RAS, related to cell proliferation, revealed a higher level in HF two-cell embryos. First cleavage rate, blastocyst cell number and division number were also higher in HF. In addition, by using a model based on polyspermy embryos, we demonstrated an increase in miR-216b levels in zygotes associated with sperm cell entry. Our results shed light on a possible mechanism of paternal contribution involving sperm-borne miR-216b that modulates levels of miR-216b in zygotes and K-RAS in two-cell embryos. This modulation might regulate early development by interfering with the first cleavage and blastocyst quality. (AU)

FAPESP's process: 16/05395-1 - Bull semen and embryos microRNAs profile and these correlation with fertility
Grantee:Eneiva Carla Carvalho Celeghini
Support Opportunities: Regular Research Grants
FAPESP's process: 15/09154-6 - Profile of microRNAs present in semen and embryos and their relationship to fertility
Grantee:Maíra Bianchi Rodrigues Alves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/23243-4 - MicroRNA profile analysis in human and mice sperm collected from different regions of the reproductive tract
Grantee:Maíra Bianchi Rodrigues Alves
Support Opportunities: Scholarships abroad - Research Internship - Doctorate