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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isoflavones improve collagen I and glycosaminoglycans and prevent bone loss in type 1 diabetic rats

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Carbonel, A. A. F. [1] ; Vieira, M. C. [2] ; Simoes, R. S. [3] ; Lima, P. D. A. [4] ; Fuchs, L. F. P. [2] ; Girao, E. R. C. [2] ; Cicivizzo, G. P. [1] ; Sasso, G. R. S. [2] ; Carvalho de Moraes, L. O. [1] ; Soares Junior, J. M. [3] ; Baracat, E. C. [3] ; Simoes, M. J. [1] ; Girao, M. J. B. C. [2]
Total Authors: 13
[1] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Morphol & Genet, Rua Botucatu, 740 Edificio Lemos Torres, BR-04023062 Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Sao Paulo - Brazil
[3] Univ Sao Paulo FMUSP, Med Fac, Dept Obstet & Gynecol, Sao Paulo - Brazil
[4] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON - Canada
Total Affiliations: 4
Document type: Journal article
Source: CLIMACTERIC; v. 23, n. 1 JUN 2019.
Web of Science Citations: 0

Objective: The objective of this study was to evaluate the action of soy isoflavones (ISO) and 17 beta-estradiol on collagen I (CollI) and sulfated glycosaminoglycans (GAGs) in the bone matrix of diabetic rats. Methods: Sixty adult female rats (Rattus norvegicus albinus) underwent ovariectomy, and then were randomized into six groups of 10 animals each: GI, sham control ovariectomized animals; GII, sham control diabetic (DM) ovariectomized animals; GIII, control ovariectomized animals receiving propylene glycol vehicle; GIV, control ovariectomized DM animals receiving propylene glycol vehicle; GV, ovariectomized DM animals treated with ISO (150 mg/kg by gavage); and GVI, ovariectomized DM animals treated with estrogen (17 beta-estradiol, 10 mg/kg, subcutaneously). 17 beta-Estradiol was used as a positive control when compared with ISO. To obtain significant depletion of the estrogen levels and subsequent bone loss, a postsurgical period of 90 days was observed. Treatments occurred during 30 consecutive days. After euthanasia, shafts of the animals' femurs were immersed in liquid nitrogen for molecular biology analysis, and the distal femurs were removed and processed for paraffin embedding. Results: ISO (GV) and 17 beta-estradiol (GVI) improved bone formation, increasing GAGs and CollI formation when compared to the control group (GIV) (p < 0.05). Conclusions: ISO and 17 beta-estradiol contribute to the decrease of bone loss in diabetic rats. (AU)

FAPESP's process: 14/17077-9 - Effects of isoflavones or 17beta-estradiol on cell-matrix interactions in the endometrium of diabetic rats: relevance for hormone therapy
Grantee:Adriana Aparecida Carbonel Castro Ferreira
Support Opportunities: Scholarships in Brazil - Post-Doctoral