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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells

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Caiaffa, Karina S. [1, 2] ; Basso, Fernanda. G. [3] ; Santos-Filho, Norival A. [4, 5] ; de Souza-Costa, Carlos Alberto [3] ; Sakai, Vivien T. [6] ; Cilli, Eduardo M. [5] ; Duque, Cristiane [1]
Total Authors: 7
[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, R Jose Bonifacio, 1193, BR-16015050 Aracatuba, SP - Brazil
[2] State Univ Sao Paulo UNESP, Aracatuba Sch Dent, Dept Endodont, Aracatuba, SP - Brazil
[3] State Univ Sao Paulo UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Aracatuba, SP - Brazil
[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Biochem & Chem Technol, Aracatuba, SP - Brazil
[5] Sao Paulo State Univ UNESP, Registro Expt Campus, Registro, SP - Brazil
[6] Fed Univ Alfenas UNIFAL, Sch Dent, Dept Clin & Surg, Alfenas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 103, p. 19-25, JUL 2019.
Web of Science Citations: 0

Objectives: To evaluate the effect of analogues of cationic peptides on the viability and the expression of phenotypic and genotypic markers of dentin mineralization in MDPC-23 odontoblast-like cells. Materials and methods: Cells were exposed to serial dilutions of analogues of cationic peptides hBD-3-1e and KR-12-a5 compared to peptide LL-37 and their viability was assessed by methyltetrazolium assay. Next, peptides (0.78-62.5 mu g/mL) were applied on the MDPC-23 cells for evaluating the total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition. Gene expression of mineralization markers (DSPP and DMP-1) was also determined by quantitative PCR. Results: LL-37 and hBD-3-1C(V) treatment did not affect cellular viability at concentrations below 62.5 mu g/mL. KR-12-a5 reduced cell viability above 31.25 mu g/mL. TP production was similar for all groups compared with the control group, except by hBD-3-1C(V) (at 15.62 mu g/mL). LL-37 (at 62.5 mu g/mL) induced higher ALP activity than control and other experimental groups. LL-37 and hBD-3-1C(V), at 62.5 mu g/mL and KR-12-a5 at 31.25 mu g/mL stimulated the highest deposition of mineralized nodule. Overall, no statistical differences were observed between the groups for DSPP-1 and DMP-1 expressions. Conclusions: LL-37 was the only peptide that induced both ALP activity and mineralized nodules deposition, without affecting cell viability. None of peptides tested induced the expression of DSPP or DMP-1, genes commonly involved in active dentin mineralization. (AU)

FAPESP's process: 13/24606-5 - Antimicrobial effect of cationic peptides analogs on endodontic microrganisms and its influence on gene expression of dentin mineralization markers
Grantee:Karina Sampaio Caiaffa
Support type: Scholarships in Brazil - Master