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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components

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de Avila, Erica D. [1, 2] ; Castro, Antonio G. B. [1] ; Tagit, Oya [3, 4] ; Krom, Bastiaan P. [5, 6] ; Lowik, Dennis [7] ; van Well, Ad A. [8] ; Bannenberg, Lars J. [8] ; Vergani, Carlos Eduardo [2] ; van den Beucken, Jeroen J. J. P. [1]
Total Authors: 9
[1] Radhoudumc, Regenerat Biomat, Philips van Leydenlaan 25, Nijmegen - Netherlands
[2] Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent Araraquara, Humaita 1680, Araraquara, SP - Brazil
[3] Radhoudumc, Dept Tumor Immunol, Geert Grootepl Zuid 28, Nijmegen - Netherlands
[4] Radhoud Inst Mol Life Sci, Geert Grootepl Zuid 28, Nijmegen - Netherlands
[5] Univ Amsterdam, Dept Prevent Dent, Acad Ctr Dent Amsterdam ACTA, Gustav Mahlerlaan 3004, Amsterdam - Netherlands
[6] Vrije Univ Amsterdam, Gustav Mahlerlaan 3004, Amsterdam - Netherlands
[7] Radhoud Univ, Dept Organ Chem, Heyendaalseweg 135, Nijmegen - Netherlands
[8] Delft Univ Technol, Fac Appl Sci, Lorentzweg 1, Delft - Netherlands
Total Affiliations: 8
Document type: Journal article
Source: Applied Surface Science; v. 488, p. 194-204, SEP 15 2019.
Web of Science Citations: 1

Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm +/- 30) and uncoated Ti discs (115 nm +/- 40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 degrees C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/ inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region. (AU)

FAPESP's process: 15/03567-7 - Modification of the physicochemical properties with different coating materials of titanium implant abutment surfaces and evaluation of their effects on the development of a polymicrobial community representative of the oral cavity: an in vitro preliminar
Grantee:Érica Dorigatti de Avila
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/19650-3 - Functional layer-by-layer coatings for implant abutment surfaces: investigating the effect of controlled release of antibacterial agents on cytokine signaling and production of inflammatory mediators induced by Porphyromonas gingivalis LPS
Grantee:Érica Dorigatti de Avila
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor