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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with Caries

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Author(s):
Picco, Daniele C. R. [1] ; Marangoni-Lopes, Lenita [1] ; Parisotto, Thais M. [2] ; Mattos-Graner, Renata [3] ; Nobre-dos-Santos, Marines [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Pediat Dent Dept, Piracicaba Dent Sch, 901 Limeira Av, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Sao Francisco, Lab Microbiol & Mol Biol, 218 Sao Francisco Assis Av, BR-12916900 Braganca Paulista, SP - Brazil
[3] Univ Estadual Campinas, Microbiol Dept, Piracicaba Dent Sch, 901 Limeira Av, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 11 JUN 1 2019.
Web of Science Citations: 0
Abstract

This study investigated pH, activity and concentration of carbonic anhydrase VI (CA VI) in dental biofilm of caries and caries-free children of 7-9 years old. Seventy-four children were selected and divided into two groups. The caries diagnosis was performed according to the WHO criteria, including the early caries lesion. After biofilm collection and pH determination, CA VI concentration and activity were determined by ELISA and Zimography respectively. The data were submitted to a Mann-Whitney test and to Pearson and Spearman correlation analyses. Means and standard deviations of dental caries for the caries group were of 3.162 +/- 1.385. The biofilm pH was significantly higher in the caries-free group. The CA VI activity was significantly higher in biofilm of children with caries. The CA VI concentration was significantly higher in biofilm of caries-free children. In caries-free children, there was a moderate negative correlation between CA VI activity and concentration in dental biofilm as well as between pH and CA VI activity. A negative correlation between biofilm pH and CA VI concentration was found in the caries group. In conclusion, CA VI was shown to be more active in the biofilm of school children with caries in order to contribute to neutralization of biofilm acid. (AU)

FAPESP's process: 12/02886-3 - Analysis of concentration and activity of carbonic anhydrase VI (CA VI) in saliva and dental plaque in children of school age.
Grantee:Daniele de Cassia Rodrigues Picco
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/15834-1 - Analysis of concentration and activity of carbonic anhydrase VI (Ca VI) in saliva and plaque in deciduous teeth and its relationship to dental caries: longitudinal study in children 3-6 years and transverse in children 7-9 years
Grantee:Marines Nobre dos Santos Uchôa
Support type: Regular Research Grants