Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Screening of Strongyloides infection using an ELISA test in transplant candidates

Full text
Author(s):
Show less -
Toledo, Beatriz [1] ; Corral, Marcelo A. [1] ; Meisel, Dirce Mary C. L. [1] ; Gottardi, Maiara [1] ; Abdala, Edson [2] ; Costa, Silvia F. [2] ; Pierrotti, Ligia Camera [2] ; Lescano, Susana A. Z. [3, 1] ; Goncalves, Elenice M. N. [4] ; Castilho, Vera L. P. [4] ; Chieffi, Pedro P. [5] ; Gryschek, Ronaldo C. B. [3, 1] ; Paula, Fabiana M. [3, 1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med LIM 06, Lab Imunopatol Esquistossom, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Secao Parasitol, Div Lab Cent, Sao Paulo, SP - Brazil
[5] Fac Ciencias Med, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Clinics; v. 74, 2019.
Web of Science Citations: 0
Abstract

OBJECTIVES: Hyperinfection or disseminated strongyloidiasis has been frequently reported after transplants and is related to high mortality. This study aimed to screen for strongyloidiasis using serological diagnoses in transplant candidates. METHODS: An ELISA test was performed with filariform larvae of Strongyloides venezuelensis as a source of antigen. RESULTS: In the serum from transplant candidates, anti-Strongyloides IgG antibodies were detected in 35/150 (23.3%) samples by soluble fractions in phosphate buffered saline (PBS), 31/150 (20.7%) samples by soluble fractions in Tris-HCl, 27/150 (18.0%) samples by membrane fractions in PBS and 22/150 (14.7%) samples by membrane fractions in Tris-HCl. CONCLUSIONS: The present results suggest the ELISA test, ideally using soluble fractions of filariform larvae S. venezuelensis in PBS, as an additional strategy for the diagnosis of strongyloidiasis in transplant candidates. (AU)

FAPESP's process: 13/04236-9 - Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis
Grantee:Ronaldo Cesar Borges Cryschek
Support Opportunities: Regular Research Grants
FAPESP's process: 10/51110-2 - Investigation of diagnostic techniques for identification of infection S. stercoratis in patients with different forms of immunosuppression
Grantee:Pedro Paulo Chieffi
Support Opportunities: Regular Research Grants