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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polyalthic Acid in Polymeric Nanoparticles Causes Selective Growth Inhibition and Genotoxicity in MCF-7 Cells

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Chibas, Leniher Castan [1] ; Cintra, Priscila Pavini [1] ; Moreira, Monique Rodrigues [1] ; Goulart, Mirian Oliveira [1] ; Ambrosio, Sergio Ricardo [1] ; Sola Veneziani, Rodrigo Cassio [1] ; Bastos, Jairo Kenupp [2] ; dos Santos, Raquel Alves [1]
Total Authors: 8
[1] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, Ave Dr Armando Salles de Oliveira 201, BR-14404600 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Polyalthic acid (PA) is a diterpene present in several trees of the Copaifera genus, with reported antitumor activity but poor water solubility. The aim of this work was the incorporation of PA in polymeric nanoparticles and the evaluation of the antiproliferative activity of this formulation in tumor (MCF-7) and normal (MCF-10A) breast cell lines. The nanoparticles were obtained by nanoprecipitation, using poly lactic-co-glycolic acid (PLGA) as the main material. Scanning electron microscopy showed nanoparticles with semispherical morphology, and dynamic light scattering measures revealed negative surface charge and average size of 98.64 +/- 28 nm. The encapsulation efficiency was 98% and the drug loading was 15.6% +/- 0.02%. Treatments with PA nanoparticles reduced cell proliferation more efficiently than free PA and the effect was selective on MCF-7 cells. Comet assay revealed a selective DNA damage induction by the nanoformulation on the tumor cells, which probably caused the antiproliferative effect. Our results show that PA incorporated in PLGA nanoparticles has potential as a selective cytostatic and genotoxic agent against MCF-7 cells. (AU)

FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/12465-0 - Assessment of DNA damage response pathways modulated by structuraly-modified stilbenes
Grantee:Raquel Alves dos Santos
Support type: Regular Research Grants