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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diversity of strategies used by atypical enteropathogenic Escherichia coli to induce attaching and effacing lesion in epithelial cells

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Author(s):
Vieira, Melissa A. [1] ; Dias, Regiane C. B. [1] ; dos Santos, Luis F. [2] ; Rall, Vera L. M. [1] ; Gomes, Tania A. T. [3] ; Hernandes, Rodrigo T. [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Microbiol & Imunol, Botucatu, SP - Brazil
[2] IAL, Ctr Bacteriol, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Medical Microbiology; v. 68, n. 6, p. 940-951, JUN 2019.
Web of Science Citations: 0
Abstract

Purpose. This study aimed to characterize 82 atypical enteropathogenic Escherichia coli (aEPEC) isolates, obtained from patients with diarrhea in Brazil, regarding their adherence patterns on HeLa cells and attaching and effacing (AE) lesion pathways. Methodology. The adherence and fluorescence-actin staining (FAS) assays were performed using HeLa cells. AE lesion pathways were determined through the detection of tyrosine residue 474 (Y-474) phosphorylation in the Tir protein, after its translocation to host cells, and by PCR assays for tir genotyping and detection of Tir-cytoskeleton coupling protein (tccP) genes. Results. Regarding the adherence pattern, determined in the presence of d-mannose, 12 isolates (14.6 %) showed the localized adherence (LA)-like pattern, 3 (3.7 %) the aggregative adherence pattern and 4 (4.9 %) a hybrid LA/diffuse adherence pattern. In addition, 36 (43.9 %) isolates displayed an undefined adherence, and 26 (31.7 %) were non-adherent (NA), while one (1.2 %) caused cell detachment. Among the 26 NA aEPEC isolates, 11 showed a type 1 pilus-dependent adherence in assays performed without d-mannose, while 15 remained NA. Forty-eight (58.5 %) aEPEC were able to trigger F-actin accumulation underneath adherent bacteria (FAS-positive), which is an important feature of AE lesions. The majority (58.3 %) of these used the Tir-Nck pathway, while 39.6 % may use both Tir-Nck and Tir-TccP pathways to induce AE lesions. Conclusion. Our results reveal the diversity of strategies used by aEPEC isolates to interact with and damage epithelial host cells, thereby causing diarrheal diseases. (AU)

FAPESP's process: 12/19077-0 - Prevalence and phenotypic and molecular characterization of Diarrheagenic Escherichia coli isolated from children with and without diarrhea in Botucatu, São Paulo
Grantee:Rodrigo Tavanelli Hernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 15/26207-6 - Investigation of the interaction strategies of an atypical enteropathogenic Escherichia coli strain displaying the hybrid localized/aggregative adherence pattern, isolated during a diarrheal outbreak in Brazil
Grantee:Rodrigo Tavanelli Hernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 13/23414-5 - Characterization and analysis of the virulence profile of atypical enteropathogenic Escherichia coli strains isolated from outbreaks and sporadic cases of diarrhea in different geographical regions of Brazil
Grantee:Melissa Arruda Vieira
Support Opportunities: Scholarships in Brazil - Master