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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Triiodothyronine (T-3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7

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Author(s):
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Silva, Tabata M. [1] ; Moretto, Fernanda C. F. [1] ; De Sibio, Maria T. [1] ; Goncalves, Bianca M. [1] ; Oliveira, Miriane [1] ; Olimpio, Regiane M. C. [1] ; Oliveira, Diego A. M. [2] ; Costa, Sarah M. B. [1] ; Depra, Igor C. [1] ; Namba, Vickeline [1] ; Nunes, Maria T. [3] ; Nogueira, Celia R. [1]
Total Authors: 12
Affiliation:
[1] Univ Estadual Paulista, Fac Med Botucatu, Dept Med Interna, UNESP, Av Prof Mario Rubens Guimaraes Montenegro S-N, BR-18618687 Botucatu, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Fisiol & Biofis, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ENDOCRINOLOGY METABOLISM; v. 63, n. 2, p. 142-147, MAR-APR 2019.
Web of Science Citations: 1
Abstract

Objective: To verify the physiological action of triiodothyronine T-3 on the expression of transforming growth factor alpha (TGFA) mRNA in MCF7 cells by inhibition of RNA Polymerase II and the MAPK/ERK pathway. Materials and methods: The cell line was treated with T-3 at a physiological dose (10(-9) M) for 10 minutes, 1 and 4 hour (h) in the presence or absence of the inhibitors, a-amanitin (RNA polymerase II inhibitor) and PD98059 (MAPK/ERK pathway inhibitor). TGFA mRNA expression was analyzed by RT-PCR. For data analysis, we used ANOVA, complemented with the Tukey test and Student t-test, with a minimum significance of 5%. Results: T-3 increases the expression of TGFA mRNA in MCF7 cells in 4 h of treatment. Inhibition of RNA polymerase II modulates the effect of T-3 treatment on the expression of TGFA in MCF7 cells. Activation of the MAPK/ERK pathway is not required for; to affect the expression of TGFA mRNA. Conclusion: Treatment with a physiological concentration of T-3 after RNA polymerase II inhibition altered the expression of TGFA. Inhibition of the MAPK/ERK pathway after T-3 treatment does not interfere with the TGFA gene expression in a breast adenocarcinoma cell line. (AU)

FAPESP's process: 14/15209-5 - PARTICIPATION OF T3 IN POST-TRANSCRIPTIONAL CONTROL OF HIF-1± AND TGF± EXPRESSION IN CELL LINES OF BREAST CANCER AND EVALUATION OF THE INVOLVEMENT OF POTENTIAL miRNAS
Grantee:Fernanda Cristina Fontes Moretto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives
Grantee:Maria Tereza Nunes
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/09686-8 - Mechanism of action of triiodothyronine hornone (T3) in the TGFa gene expression in breast adenocarcinoma cell lines
Grantee:Tabata Marinda da Silva
Support type: Scholarships in Brazil - Scientific Initiation