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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins

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Author(s):
de Souza, Caroline Lacerra [1] ; Aparecido dos Santos-Pinto, Jose Roberto [1] ; Esteves, Franciele Grego [1] ; Perez-Riverol, Amilcar [1] ; Romani Fernandes, Luis Gustavo [2] ; Zollner, Ricardo de Lima [2] ; Palma, Mario Sergio [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, BR-13500 Rio Claro, SP - Brazil
[2] Univ Campinas UNICAMP, Fac Med, Lab Translat Immunol, Cidade Univ Zeferino Vaz, BR-13083887 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 200, p. 60-73, MAY 30 2019.
Web of Science Citations: 2
Abstract

The partial proteome of Polybia paulista wasp venom was previously reported elsewhere using a gel-dependent approach and resulted in the identification of a limited number of venom toxins. Here, we reinvestigated the P. paulista venom using a gel-free shotgun proteomic approach; the highly dynamic range of this approach facilitated the detection and identification of 1673 proteins, of which 23 venom proteins presented N-linked glycosylation as a posttranslational modification. Three different molecular forms of PLAT were identified as allergenic proteins, and two of these forms were modified by N-linked glycosylation. This study reveals an extensive repertoire of hitherto undescribed proteins that were classified into the following six different functional groups: (i) typical venom proteins; (ii) proteins related to the folding/conformation and PTMs of toxins; (iii) proteins that protect toxins from oxidative stress; (iv) proteins involved in chemical communication; (v) housekeeping proteins; and (vi) uncharacterized proteins. It was possible to identify venom toxin-like proteins that are commonly reported in other animal venoms, including arthropods such as spiders and scorpions. Thus, the findings reported here may contribute to improving our understanding of the composition of P. paulista venom, its envenoming mechanism and the pathologies experienced by the victim after the wasp stinging accident. Biological significance: The present study significantly expanded the number of proteins identified in P. paulista venom, contributing to improvements in our understanding of the envenoming mechanism produced by sting accidents caused by this wasp. For example, novel wasp venom neurotoxins have been identified, but no studies have assessed the presence of this type of toxin in social wasp venoms. In addition, 23 N-linked glycosylated venom proteins were identified in the P. paulista venom proteome, and some of these proteins might be relevant allergens that are immunoreactive to human IgE. (AU)

FAPESP's process: 17/22405-3 - IDENTIFICATION AND SYNTHESIS OF PEPTIDES CORRESPONDING TO B-CELL LINEAR EPITOPES IN ALLERGENS FROM VENOM OF SOCIAL HYMENOPTERA: DEVELOPMENT OF SUPPLIES FOR DIAGNOSIS AND IMMUNOTHERAPY OF ALLERGY
Grantee:Amilcar Perez Riverol
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/10373-0 - Profiling the peptidomic and structural-functional characterization of lipid vesicles present in the Nephila clavipes web spider
Grantee:Franciele Grego Esteves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/04680-7 - Glicoproteomic analysis of venom from the social wasp Polybia paulista
Grantee:Caroline Lacerra de Souza
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/26451-9 - Bioprospecting and Structural Analysis of the Silk Proteins of Arthropods by a Proteomics Approach Using nanoLC-ESI-CID/ETD System
Grantee:José Roberto Aparecido dos Santos-Pinto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/16212-5 - Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays
Grantee:Mario Sergio Palma
Support type: BIOTA-FAPESP Program - Thematic Grants