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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool

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Author(s):
de Borba, Rafael Splendore [1] ; Mariotto, Sandra [2] ; Centofante, Liano [3] ; Zawadzki, Claudio Henrique [4] ; Parise-Maltempi, Patricia Pasquali [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ Unesp, Inst Biosci, Rio Claro - Brazil
[2] Mato Grosso Fed Inst IFMT, Cuiaba - Brazil
[3] Univ Fed Mato Grosso, Inst Biosci, Cuiaba - Brazil
[4] Univ Estadual Maringa, Dept Biol, Ctr Res Limnol Ichthyol & Aquaculture Nupelia, Maringa, Parana - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MITOCHONDRIAL DNA PART A; v. 30, n. 4 APR 2019.
Web of Science Citations: 0
Abstract

Although several species of Ancistrus have been described from the Amazon and Paraguay river basins in the states of Amazonas and Mato Grosso, Brazil, the taxonomic status of most specimens from these regions remains doubtful. In the present work, cytogenetic and molecular data were used to discriminate and isolate unexpected Ancistrus lineages from the Amazon and Paraguay basins. For that, it was used DNA barcoding based on mitochondrial Cytochrome Oxidase Subunit I (COI) gene and cytogenic data to perform such molecular discrimination. The analyzed sequences had 669 bp, of which 171 bp were conserved and 491 bp were variable. The Neighbor-joining and Bayesian analysis revealed 21 distinct groups in topology. The genetic distances within each group was 0.4%, 21 times smaller than the mean distance observed among groups, which was 8.4%. These values showed seven distinct lineages of Ancistrus from the studied points of the Amazon basin and eight lineages from the Paraguay basin points. Our results illustrate the efficiency of this technique for the discrimination of the Ancistrus lineages once it indicates the occurrence of cryptic species in these regions, which cannot yet be identified either with just chromosomal or morphological analyzes. (AU)

FAPESP's process: 13/17211-4 - Study of chromosomes and molecular phylogeny of the genus Ancistrus (Siluriformes: Loricariidae)
Grantee:Patricia Pasquali Parise Maltempi
Support type: Regular Research Grants
FAPESP's process: 13/17826-9 - PHYLOGENETIC AND PHYLOGEOGRAPHIC RELATIONS AND MOLECULAR DISCRIMINATION OF THE ANCISTRUS GENUS SPECIES KNER, 1854 (SILURIFORMES: LORICARIIDAE) THAT COMPOSE THREE WATERSHED OF THE MATO GROSSO STATE, BRAZIL.
Grantee:Rafael Splendore de Borba
Support type: Scholarships in Brazil - Doctorate