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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Could chlorhexidine be an adequate positive control for antimicrobial photodynamic therapy in- in vitro studies?

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Chaves Lamarque, Giuliana Campos [1] ; Cusicanqui Mendez, Daniela Alejandra [1] ; Gutierrez, Eliezer [1] ; Dionisio, Evandro Jose [1] ; Andrade Moreira Machado, Maria Aparecida [1] ; Oliveira, Thais Marchini [1] ; Rios, Daniela [1] ; Cruvinel, Thiago [1]
Total Authors: 8
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 25, p. 58-62, MAR 2019.
Web of Science Citations: 2

Background: Chlorhexidine digluconate (CHX) is commonly applied as positive control of new antimicrobials, because it is considered the gold-standard for chemical plaque control. The aim of this study was to compare the effect of treatments with curcumin-mediated aPDT and CHX in relation to the viability of specific microorganism groups in two distinct times (immediately and 24 h later). Methods: Dentin caries microcosms were grown on bovine dentin discs (37 degrees C, anaerobiosis) for 3 days in the Active Attachment Amsterdam Biofilm Model. The biofilms were treated with 300 mu M curcumin and 75 LED, or 0.06% and 0.12% CHX. Then, total microorganisms, total streptococci, mutans streptococci, and total lactobacilli counts were determined. The statistical analysis was conducted by Kruskal-Wallis and post-hoc Dunn's tests (P < 0.05). Results: Curcumin-mediated aPDT (C + L + ), 0.06% and 0.12% CHX reduced mutans streptococci counts (0.19, 0.10 and 0.07 log10 respectively) in the immediate analysis. After 24 h, it was observed a re-growth of microorganisms treated by curcumin-mediated aPDT, whereas both CHX concentrations demonstrated a decrease of the viable microorganisms. Conclusion: This study confirmed the substantive effect of CHX and the immediate effect of aPDT. The use of a neutralizer solution was important to block the substantivity of CHX and permit its fair comparison with aPDT, allowing its use as a positive control in further studies. (AU)

FAPESP's process: 14/10897-0 - Effect of antimicrobial photodynamic therapy on dentin caries microcosms
Grantee:Thiago Cruvinel da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 16/24962-4 - Comparison between the antimicrobial effects of Photodynamic Antimicrobial Therapy mediated by curcumin and chlorhexidine on microcosms of dentin caries lesions
Grantee:Eliézer Gutierres
Support Opportunities: Scholarships in Brazil - Scientific Initiation