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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comprehensive Genomic Profiling of Androgen-Receptor-Negative Canine Prostate Cancer

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Laufer-Amorim, Renee [1] ; Fonseca-Alves, Carlos Eduardo [2] ; Rios Villacis, Rolando Andre [3] ; Drigo Linde, Sandra Aparecida [1] ; Carvalho, Marcio [1] ; Larsen, Simon Jonas [4] ; Marchi, Fabio Albuquerque [5] ; Rogatto, Silvia Regina [6]
Total Authors: 8
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, BR-18680970 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, BR-18680970 Botucatu, SP - Brazil
[3] Univ Brasilia UnB, Inst Biol Sci, Dept Genet & Morphol, BR-70910900 Brasilia, DF - Brazil
[4] Univ Southern Denmark, Dept Math & Comp Sci, DK-5230 Odense - Denmark
[5] AC Camargo Canc Ctr, Int Res Ctr CIPE, BR-01508010 Sao Paulo - Brazil
[6] Univ Southern Denmark, Vejle Hosp, Inst Reg Hlth Res, Dept Clin Genet, DK-7100 Vejle - Denmark
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 1

Canine carcinomas have been considered natural models for human diseases; however, the genomic profile of canine prostate cancers (PCs) has not been explored. In this study, 14 PC androgen-receptor-negative cases, 4 proliferative inflammatory atrophies (PIA), and 5 normal prostate tissues were investigated by array-based comparative genomic hybridization (aCGH). Copy number alterations (CNAs) were assessed using the Canine Genome CGH Microarray 4 x 44K (Agilent Technologies). Genes covered by recurrent CNAs were submitted to enrichment and cross-validation analysis. In addition, the expression levels of TP53, MDM2 and ZBTB4 were evaluated in an independent set of cases by qPCR. PC cases presented genomic complexity, while PIA samples had a small number of CNAs. Recurrent losses covering well-known tumor suppressor genes, such as ATM, BRCA1, CDH1, MEN1 and TP53, were found in PC. The in silico functional analysis showed several cancer-related genes associated with canonical pathways and interaction networks previously described in human PC. The MDM2, TP53, and ZBTB4 copy number alterations were translated into altered expression levels. A cross-validation analysis using The Cancer Genome Atlas (TCGA) database for human PC uncovered similarities between canine and human PCs. Androgen-receptor-negative canine PC is a complex disease characterized by high genomic instability, showing a set of genes with similar alterations to human cancer. (AU)

FAPESP's process: 15/25400-7 - In vitro and molecular characterization of canine prostatic cells and evaluation of antitumor response against target drugs
Grantee:Carlos Eduardo Fonseca Alves
Support type: Scholarships in Brazil - Post-Doctorate