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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama

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Author(s):
Kadir González ; Rosendo Diaz [2] ; Aurea F. Ferreira [3] ; Víctor García [4] ; Héctor Paz [5] ; José E. Calzada [6] ; Michelle Ruíz [7] ; Márcia Laurenti [8] ; Azael Saldaña
Total Authors: 9
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 60, 2018-02-15.
Abstract

ABSTRACT Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulomatous, with the presence of lymphocytes, plasma cells and macrophages. This study describes the histopathological characteristics found in the skin lesions of patients with CL caused by Leishmania (V.) panamensis in Panama. We analyzed 49 skin biopsy samples from patients with clinical suspicion of CL, by molecular tests (PCR for subgenus Viannia and HSP-70) and by Hematoxylin-Eosin staining. Samples were characterized at the species level by PCR-HSP-70/RFLP. From the 49 samples studied, 46 (94%) were positive by PCR and were characterized as Leishmania (V.) panamensis. Of these, 48% were positive by Hematoxylin-Eosin staining with alterations being observed both, in the epidermis (85%) and in the dermis (100%) of skin biopsies. The inflammatory infiltrate was characterized according to histopathological patterns: lymphohistiocytic (50%), lymphoplasmacytic (61%) and granulomatous (46%) infiltration, being the combination of these patterns frequently found. The predominant histopathological characteristics observed in CL lesions caused by L. (V.) panamensis in Panama were: an intense inflammatory reaction in the dermis with a combination of lymphohistiocytic, lymphoplasmacytic and granulomatous presentation patterns and the presence of ulcers, acanthosis, exocytosis and spongiosis in the epidermis. (AU)

FAPESP's process: 17/03141-5 - Characterization of histopathological changes and cellular immune response in cutaneous lesions of patients with Tegumentary Leishmaniasis in Panamá
Grantee:Kadir Amilcar Gonzalez Carrion
Support type: Scholarships in Brazil - Doctorate (Direct)