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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae)

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Author(s):
Bordon, Isabella C. [1] ; de Campos, Bruno Galvao [1] ; Gusso-Choueri, Paloma Kachel [1] ; Miyai, Caio Akira [2] ; de Araujo, Giuliana Seraphim [1] ; Emerenciano, Andrews Krupinski [3] ; Machado Cunha da Silva, Jose Roberto [3] ; Barboza Cotrim, Marycel Elena [4] ; Teixeira Favaro, Deborah Ines [5] ; de Souza Abessa, Denis Moledo [1]
Total Authors: 10
Affiliation:
[1] UNESP Univ Estadual Paulista, NEPEA, Campus Litoral Paulista, BR-11330900 Sao Vicente, SP - Brazil
[2] UNESP Univ Estadual Paulista, Lab Ecol & Comportamento Anim Labecom, Campus Litoral Paulista, BR-11330900 Sao Vicente, SP - Brazil
[3] Univ Sao Paulo, Biomed & Biol Sci Inst ICB, Av Prof Lineu Prestes 1524, BR-05508900 Sao Paulo, SP - Brazil
[4] IPEN Nucl & Energy Res Inst, Chem & Environm Technol Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo, SP - Brazil
[5] IPEN Nucl & Energy Res Inst, Res Reactor Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: International Journal of Environmental Research; v. 13, n. 2, p. 431-434, APR 2019.
Web of Science Citations: 0
Abstract

This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14days, respectively. After confirming that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 mu g g(-1) were prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were > 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial foods (0.5 and 2.0 mu g g(-1) of Pb) during 7 and 14days, respectively. The observed acceptance of these foods by crabs at both Pb concentrations was of 100%. Article HighlightsAn artificial food was prepared to assess bioaccumulation in the blue crabLead (Pb) was chosen as the metal of interestThe acceptance of artificial food was evaluatedIn the contaminated artificial foods, Pb was determinedIt was concluded that this food can be applied in metal exposure assays (AU)

FAPESP's process: 14/01576-6 - Assessment of the effects of exposure to Pb through respiration and feeding in blue crabs Callinectes danae
Grantee:Isabella Cristina da Costa Leal Bordon
Support type: Scholarships in Brazil - Post-Doctorate