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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill.) N. E. Brown and Biological Activities

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de Jesus, Raphael A. [1] ; Prado, Vilma M. J. [1] ; Pinto, Vanderson S. [2] ; Silva, Valdenizia R. [3] ; Santos, Luciano S. [3] ; Nogueira, Paulo C. L. [1] ; Navickiene, Sandro [1] ; Pereira-Filho, Edenir R. [4] ; Blank, Arie E. [2] ; Bezerra, Daniel P. [3] ; Soares, Milena B. P. [5, 3] ; Seidl, Claudia [6] ; Cardoso, Carmen L. [6] ; Moraes, Valeria R. S. [1]
Total Authors: 14
[1] Univ Fed Sergipe, Dept Quim, Av Marechal Rondon S-N, BR-49100000 Sao Cristovao, SE - Brazil
[2] Univ Fed Sergipe, Dept Engn Agron, Av Marechal Rondon S-N, BR-49100000 Sao Cristovao, SE - Brazil
[3] Fundacao Oswaldo Cruz, Inst Goncalo Moniz, Rua Waldemar Falcao 121, BR-40296710 Salvador, BA - Brazil
[4] Univ Fed Sao Carlos, Dept Quim, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[5] Hosp Sao Rafael, Ctr Biotecnol & Terapia Celular, Ave Sao Rafael 2152, BR-41253190 Salvador, BA - Brazil
[6] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 30, n. 5, p. 978-987, MAY 2019.
Web of Science Citations: 0

The methodology developed in this study through the acquisition of fingerprint chromatograms by liquid chromatography-diode array detector (LC-DAD), aided by principal component analysis, made it possible to assess, in a rational way, the chemical differences between six accessions of Lippia alba and to verify the influence of the method of extraction, the seasonality and the individuality of each accession on chemical composition of its extracts. Among all extracts analyzed against cancer cell lines, eight of them showed to be more promising against a human leukemia cell line (HL-60), displaying cell growth inhibition percentage ranging between 40.0 and 52.0%. In the inhibitory activity assays against acetylcholinesterase enzyme, all extracts showed weak inhibitory effect, with highlight only for six of them, which displayed inhibition ranging between 27.0 and 32.0%. (AU)

FAPESP's process: 13/01710-1 - Enzyme ligand: new models of screening
Grantee:Quezia Bezerra Cass
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/11640-3 - LC-MS / 2D methods for screening ligands for evaluating enzyme inhibitory activity and identification of active compounds in crude extracts
Grantee:Cláudia Seidl
Support Opportunities: Scholarships in Brazil - Post-Doctoral