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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melatonin and Leishmania amazonensis Infection Altered miR-294, miR-30e, and miR-302d Impacting on Tnf, Mcp-1, and Nos2 Expression

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Ribeiro Fernandes, Juliane Cristina [1, 2] ; Aoki, Juliana Ide [1] ; Acuna, Stephanie Maia [1] ; Zampieri, Ricardo Andrade [1] ; Markus, Regina P. [1] ; Floeter-Winter, Lucile Maria [1] ; Muxel, Sandra Marcia [1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Med Trop, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 2

Leishmaniases are neglected diseases that cause a large spectrum of clinical manifestations, from cutaneous to visceral lesions. The initial steps of the inflammatory response involve the phagocytosis of Leishmania and the parasite replication inside the macrophage phagolysosome. Melatonin, the darkness-signaling hormone, is involved in modulation of macrophage activation during infectious diseases, controlling the inflammatory response against parasites. In this work, we showed that exogenous melatonin treatment of BALB/c macrophages reduced Leishmania amazonensis infection and modulated host microRNA (miRNA) expression profile, as well as cytokine production such as IL-6, MCP-1/CCL2, and, RANTES/CCL9. The role of one of the regulated miRNA (miR-294-3p) in L. amazonensis BALB/c infection was confirmed with miRNA inhibition assays, which led to increased expression levels of Tnf and Mcp-1/Ccl2 and diminished infectivity. Additionally, melatonin treatment or miR-30e-5p and miR-302d-3p inhibition increased nitric oxide synthase 2 (Nos2) mRNA expression levels and nitric oxide (NO) production, altering the macrophage activation state and reducing infection. Altogether, these data demonstrated the impact of melatonin treatment on the miRNA profile of BALB/c macrophage infected with L. amazonensis defining the infection outcome. (AU)

FAPESP's process: 16/19815-2 - Role of miRNAs in the TLRs-mediated immune response to Leishmania amazonensis infection
Grantee:Sandra Marcia Muxel
Support Opportunities: Regular Research Grants
FAPESP's process: 16/03273-6 - Dual transcriptome profiling of murine macrophages infected with Leishmania amazonensis
Grantee:Juliana Ide Aoki
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/15263-4 - Alterations in the composition of microRNAs in macrophages infected by Leishmania amazonensis
Grantee:Sandra Marcia Muxel
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50717-1 - Biochemical, physiological and functional genomic studies of Leishmania-macrophage interaction
Grantee:Lucile Maria Floeter-Winter
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/23519-2 - Analysis of role of miRNAs and transcription factors on the regulation of gene expression of Leishmania amazonensis infected murine macrophages
Grantee:Stephanie Maia Acuna
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)