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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intravaginal Delivery of Syngonanthus nitens (Bong.) Ruhland Fraction Based on a Nanoemulsion System Applied to Vulvovaginal Candidiasis Treatment

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dos Santos Ramos, Matheus Aparecido [1] ; da Silva, Patricia Bento [2] ; de Toledo, Luciani Gaspar [1] ; Oda, Fernando Bombarda [3] ; da Silva, Isabel Cristiane [1] ; dos Santos, Lourdes Campaner [4] ; dos Santos, Andre Gonzaga [3] ; Gottardo de Almeida, Margarete Teresa [5] ; Pavan, Fernando Rogerio [1] ; Chorilli, Marius [2] ; Bauab, This Maria [1]
Total Authors: 11
[1] Sao Paulo State Univ UNESP, Dept Biol Sci, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil
[2] Sao Paulo State Univ UNESP, Dept Drug & Med, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Nat Prod & Toxicol, Sch Pharmaceut Sci, Campus Araraquara, BR-14800903 Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ UNESP, Dept Organ Chem, Chem Inst Campus Araraquara, BR-14800060 Sao Paulo, SP - Brazil
[5] Med Sch Sao Jose Rio Preto FAMERP, Dept Infect Dis, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY; v. 15, n. 5, p. 1072-1089, MAY 2019.
Web of Science Citations: 0

In this study, was evaluated the chemical composition of a fraction from Syngonanthus nitens extract and its antimicrobial potential unloaded (Fr3) and loaded (F9Fr3) into a nanoemulsion (F9) composed of cholesterol as the oil phase (10%), polyoxyethylene 20-cetyl ether and soy phosphatidylcholine (2:1) as surfactant (20%), and a solution of phosphate buffer (pH 7.4) plus chitosan polymer dispersion (0.25%) as the aqueous phase (70%) to use for VVC treatment. Phytochemical procedures showed that Fr3 is rich in luteolin, which is responsible for the antimicrobial activity. F9 development showed satisfactory parameters for use in the vulvovaginal candidiasis (VVC) treatment, as F9 demonstrated pseudoplastic, elastic behavior, and adhesive properties on vaginal mucosa. In addition, we observed improvement in antimicrobial potential of Fr3 on planktonic and biofilms after incorporation in F9. Fr3 and F9Fr3 showed satisfactory parameters related to toxic profiles in cell lines and in a model of acute toxicity by Artemia salina. The in vivo VVC assay showed that F9Fr3 was more active than unloaded Fr3 in VVC treatment. In conclusion, this work showed that use of a fraction rich in luteolin can be a used as an antimicrobial for treatment of vaginal infections and that use of nanostructured lipid systems was an important factor in the biological efficacy of Fr3, especially in treatment of acute VVC. (AU)

FAPESP's process: 14/24626-9 - Syngonanthus nitens (Bong.) Ruhland: prospection chemico-biologic of methanolic extract of scapes in the treatment of vuvlvovaginal candidiasis.
Grantee:Matheus Aparecido dos Santos Ramos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/08559-5 - Chemical analyze and therapeutic prospection of Syngonanthus nitens (Bong.) Ruhland and Cymbopogon nardus (L.) Rendle loaded into nanotechnological systems applied to vulvovaginal candidiasis treatment
Grantee:Taís Maria Bauab
Support type: Regular Research Grants