Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Early Peritoneal CC Chemokine Production Correlates with Divergent Inflammatory Phenotypes and Susceptibility to Experimental Arthritis in Mice

Full text
Show less -
Rossato, Cristiano [1, 2] ; Albuquerque, Layra Lucy [3] ; Santos Katz, Iana Suly [4] ; Borrego, Andrea [2] ; Koury Cabrera, Wafa Hanna [2] ; Spadafora-Ferreira, Monica [2] ; Ribeiro, Orlando Garcia [2] ; Starobinas, Nancy [2] ; Ibanez, Olga Martinez [2] ; De Franco, Marcelo [2, 4] ; Jensen, Jose Ricardo [2]
Total Authors: 11
[1] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Butantan Inst, Immunogenet Lab, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
[3] Uniao Educ Norte, BR-69915901 Rio Branco - Brazil
[4] Pasteur Inst, Diagnost Sect, Ave Paulista 393, BR-01311000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 2

The inflammatory and autoimmune events preceding clinical symptoms in rheumatoid arthritis (RA) and other autoimmune diseases are difficult to study in human patients. Therefore, animal models that share immunologic and clinical features with human RA, such as pristane-induced arthritis (PIA), are valuable tools for assessing the primordial events related to arthritis susceptibility. PIA-resistant HIII and susceptible LIII mice were injected i.p. with pristane, and peritoneal lavage fluid was harvested in the early (7 days) and late (35 days) preclinical phases of PIA. Chemokine and cytokine levels were measured in lavage supernatant with ELISA, peritoneal inflammatory leukocytes were immunophenotyped by flow cytometry, and gene expression was determined by qRT-PCR. Leukocyte recruitment was quantitatively and qualitatively divergent in the peritoneum of HIII and LIII mice, with an early increase of CC chemokines (CCL2/CCL3/CCL5/CCL12/CCL22) in the susceptible LIII strain. Also, cytokines such as IL-12p40, IL-23, and IL-18 were elevated in LIII mice while IL-6 was increased in HIII animals. The results show that an early peritoneal CC chemokine response is an important feature of arthritis susceptibility and defines potential biomarkers in this model. (AU)

FAPESP's process: 12/50764-4 - Mapping of pristane induced arthritis quantitative trait loci in selection III mice
Grantee:José Ricardo Jensen
Support Opportunities: Regular Research Grants