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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular epidemiology of methicillin-resistant Staphylococcus aureus in the Brazilian primary health care system

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Author(s):
Lino Pereira-Franchi, Eliane Patricia [1] ; Nogueira Barreira, Maria Rachel [1] ; Lima Moreira da Costa, Natalia de Sousa [1] ; Moraes Riboli, Danilo Flavio [1] ; Abraao, Ligia Maria [1, 2] ; Martins, Katheryne Benini [1] ; Victoria, Cassiano [3] ; Ribeiro de Souza da Cunha, Maria de Lourdes [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias Botucatu, Dept Microbiol & Imunol, Botucatu, SP - Brazil
[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Med Botucatu, Dept Doencas Tropicais, Botucatu, SP - Brazil
[3] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: TROPICAL MEDICINE & INTERNATIONAL HEALTH; v. 24, n. 3, p. 339-347, MAR 2019.
Web of Science Citations: 0
Abstract

Objective To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units (BHUs) of a Brazilian city. Methods Observational, cross-sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterised by identifying virulence genes encoding enterotoxins A-E, haemolysins alpha, beta and delta, exfoliatins A, B and D, biofilm production, Panton-Valentine Leukocidin and toxic shock syndrome toxin 1, and by pulsed-field gel electrophoresis (PFGE), multilocus sequence and spa typing. Results Eighteen methicillin-resistant Staphylococcus aureus (MRSA) (6 SCCmec type II and 12 SCCmec type IV) and 101 (85%) MSSA were identified. PFGE typing resulted in the formation of eight clusters, with STs 1, 5, 8, 30, 188, 1176 and 1635 and spa type t002 being the predominant types among MSSA. The 18 MRSA belonged to STs 5, 8 and 1176 and spa types t002 and t062. Conclusion The results demonstrate widespread dissemination of MSSA and MRSA clones carrying haemolysin, biofilm and toxin genes. Kernel density estimation revealed the highest density of S. aureus in the 4, 5 and 8 BHUs. (AU)

FAPESP's process: 12/00257-9 - Molecular epidemiology and study of virulence factors of Staphylococcus aureus resistant to oxacillin isolated from wounds in patients treated at Basic Health Units in the city of Botucatu
Grantee:Eliane Patricia Lino Pereira Franchi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/10975-9 - Protective efficacy of active immunization with the recombinant lipoproteins of Staphylococcus aureus in an experimental model of bacteremia
Grantee:Eliane Patricia Lino Pereira Franchi
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/10146-7 - Molecular epidemiology and study of the virulence factors of Staphylococcus aureus methicillin resistant isolated from wounds in patients treated in Basic Health Units in the city of Botucatu
Grantee:Maria de Lourdes Ribeiro de Souza da Cunha
Support type: Regular Research Grants