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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of OCT3/4, Nestin, NANOG, CD44 and CD24 as stem cell markers in canine prostate cancer

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Costa, Camila Dorotea [1] ; Justo, Andre Augusto [2] ; Kobayashi, Priscila Emiko [2] ; Story, Michelle M. [3] ; Palmieri, Chiara [3] ; Amorim, Renee Laufer [2] ; Fonseca-Alves, Carlos Eduardo [1]
Total Authors: 7
[1] Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anaesthesiol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
[3] Univ Queensland, Sch Vet Sci, Gatton Campus, Gatton, Qld - Australia
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 3

The cancer cell population is heterogeneous, and cancer stem cells (CSCs) are important for tumor growth and maintenance. The CSC population is associated with different neoplastic characteristics, such as cell migration, resistance to apoptosis, radiation therapy and chemotherapy. To increase the knowledge of CSCs in canine prostate cancer (PC), we characterized CSC markers in canine PC tissues and tumorspheres. We performed immunohistochemistry of OCT3/4, Nestin, NANOG, CD44 and CD24 in 10 normal canine prostatic tissue samples, 10 prostatic hyperplastic (PH) tissue samples and 28 PC tissue samples. Then, we established two canine prostate cancer cell cultures and characterized the CSC profile of tumorspheres grown from these cultures. Normal and PH tissues were positive for Nestin, NANOG, CD44 and CD24 only in the basal cell layer. OCT3/4 was expressed in the luminal cells of normal and PH tissues. There was no significant difference in Nestin expression among the prostatic tissues. However, we found higher expression of NANOG and CD44 in canine PC tissues than that in normal and PH tissues. Tumorspheres from canine prostate cancer cells express OCT3/4, Nestin, NANOG and CD44, indicating that these markers may be potential cancer stem cell markers in canine PC. The results obtained can be useful to better characterize the stem cell population in canine prostatic cancer and to guide future studies in comparative oncology. (AU)

FAPESP's process: 17/25822-4 - Evaluation of COX-2 expression and antitumor effect of piroxicam and firocoxib on tumorspheres of canine prosthetic carcinomas and canine mammary carcinomas
Grantee:Camila Dorotea Costa
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/25400-7 - In vitro and molecular characterization of canine prostatic cells and evaluation of antitumor response against target drugs
Grantee:Carlos Eduardo Fonseca Alves
Support type: Scholarships in Brazil - Post-Doctorate