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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of hybrid polymeric nanoparticle/thermosensitive hydrogels systems on formulation tracking and in vitro artificial membrane permeation: A promising system for skin drug-delivery

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Grillo, Renato [1] ; Dias, V, Fabiana ; Querobino, Samyr M. [2] ; Alberto-Silva, Carlos [2] ; Fraceto, Leonardo F. [3] ; de Paula, Eneida [4] ; de Araujol, Daniele R. [2]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Sch Engn, Dept Phys & Chem, Ilha Solteira, SP - Brazil
[2] Dias, Fabiana, V, Fed Univ ABC UFABC, Human & Nat Sci Ctr, Santo Andre, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Environm Engn, Sorocaba, SP - Brazil
[4] Univ Estadual Campinas, Dept Biochem & Tissue Biol, UNICAMP, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 174, p. 56-62, FEB 1 2019.
Web of Science Citations: 5

In recent years, the development of hybrid drug delivery systems, such as hydrogels and nanoparticles, has gained considerable attention as new formulations for skin-delivery. Meanwhile, transdermal diffusion synthetic membranes have been used to assess skin permeability to these systems, providing key insights into the relationships between drug and nanoformulations. In this study, benzocaine-loaded poly-epsilon-caprolactone nanoparticles (BZC:NPs) were synthesized, characterized and incorporated into Poloxamer 407-based hydrogel (PL407). Benzocaine (BZC) was used as a drug model since has been commonly applied as a topical pain reliever in the last years. Hence, we developed a hybrid polymeric nanoparticle/thermosensitive hydrogels system and evaluated the in vitro permeation of the BZC, as well as nanoformulation tracking in an artificial membrane. In vitro permeation study was conducted in a vertical diffusion cell system using a Strat-M (R) membrane model. BZC:NPs were prepared by coprecipitation method and their physicochemical stability measured before incorporating into the thermosensitive hydrogel. Also, viscosity measurements and sol-gel transition temperature were performed by rheological analysis. Different techniques, including microscopy, were used to tracking the nanoparticles on both receptor medium and synthetic membranes. Results showed high BZC encapsulation efficiency into NPs (93%) and good physicochemical stability before and after hydrogel incorporation. BZC in vitro permeation kinetics from NPs-loaded Poloxamer 407-based hydrogel presented slower permeation profile compared with the BZC: Poloxamer 407-based hydrogel. Also, NPs were observed into the diffusion cells receptor compartment after the in vitro permeation study. These results contribute to a better understanding the interaction between hydrogels, nanoparticles and synthetic membrane, as well as open perspectives for the development of new drug delivery systems for skin. (AU)

FAPESP's process: 15/26189-8 - Development of nanostructured lipid carriers functionalized with hyaluronic acid for local anesthetics modified release by intra-articular route
Grantee:Renato Grillo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics
Grantee:Eneida de Paula
Support Opportunities: Research Projects - Thematic Grants