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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols

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Torres, Mariana A. [1] ; Monteiro, Matheus S. [1] ; Passarelli, Marina S. [1] ; Papa, Frederico O. [2] ; Dell'Aqua, Jr., Jose Antonio [2] ; Alvarenga, Marco Antonio [2] ; Martins, Simone M. M. K. [1] ; de Andrade, Andre F. C. [1]
Total Authors: 8
[1] Univ Sao Paulo, Swine Res Ctr, Sch Vet Med & Anim Sci, Pirassununga, SP - Brazil
[2] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CRYOBIOLOGY; v. 86, p. 58-64, FEB 2019.
Web of Science Citations: 0

Boar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h. (AU)

FAPESP's process: 15/17620-7 - The use of holding time associated with boar semen cryopreservation protocols
Grantee:Matheus Saliba Monteiro
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/24690-4 - Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance
Grantee:André Furugen Cesar de Andrade
Support Opportunities: Regular Research Grants
FAPESP's process: 16/09441-8 - Metabolomics sgnatures on the influence of holding time on increasing boar spermatozoa cryotolerance
Grantee:Mariana Andrade Torres
Support Opportunities: Scholarships in Brazil - Doctorate