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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass

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Brondi, Mariana G. [1, 2] ; Vasconcellos, Vanessa M. [1, 2] ; Giordano, Roberto C. [1] ; Farinas, Cristiane S. [1, 2]
Total Authors: 4
[1] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] EMBRAPA Instrumentacao, Rua XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 187, n. 2, p. 461-473, FEB 2019.
Web of Science Citations: 4

A potential strategy to mitigate problems related to unproductive adsorption of enzymes onto lignin during the saccharification of lignocellulosic biomass is the addition of lignin-blocking agents to the hydrolysis reaction medium. However, there is a clear need to find more cost-effective additives for use in large-scale processes. Here, selected alternative low-cost additives were evaluated in the saccharification of steam-exploded sugarcane bagasse using a commercial enzymatic cocktail. The addition of soybean protein, tryptone, peptone, and maize zein had positive effects on glucose release during the hydrolysis, with gains of up to 36% when 8% (w/w) soybean protein was used. These improvements were superior to those obtained using bovine serum albumin (BSA), a much more expensive protein that has been widely reported for such an application. Moreover, addition of soybean protein led to a saving of 48h in the hydrolysis, corresponding to a 66% decrease in the reactor operation time required. In order to achieve the same hydrolysis yield without the soybean additive, the enzyme loading would need to be increased by 50%. FTIR spectroscopy and nitrogen elemental analysis revealed that the additives probably acted to reduce unproductive binding of cellulolytic enzymes onto the lignin portion of the sugarcane bagasse. (AU)

FAPESP's process: 17/13931-3 - Soybean protein as an additive in the enzymatic hydrolysis of vegetal biomass: development and techno-economic analysis of the process
Grantee:Mariana Govoni Brondi
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/19000-3 - Simplification of the biomass to ethanol conversion process by integration with the production of enzymes in-house
Grantee:Cristiane Sanchez Farinas
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants