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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Incidence of Y-chromosome microdeletions in children whose fathers underwent vasectomy reversal or in vitro fertilization with epididymal sperm aspiration: a case-control study

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Author(s):
Milton Ghirelli-Filho [1] ; Patricia Leme de Marchi [2] ; Fernanda Abani Mafra [3] ; Viviane Cavalcanti [4] ; Denise Maria Christofolini [5] ; Caio Parente Barbosa [6] ; Bianca Bianco [7] ; Sidney Glina [8]
Total Authors: 8
Affiliation:
[1] Faculdade de Medicina do ABC - Brasil
[2] Faculdade de Medicina do ABC - Brasil
[3] Faculdade de Medicina do ABC - Brasil
[4] Faculdade de Medicina do ABC - Brasil
[5] Faculdade de Medicina do ABC - Brasil
[6] Faculdade de Medicina do ABC - Brasil
[7] Faculdade de Medicina do ABC - Brasil
[8] Hospital Israelita Albert Einstein - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Einstein (São Paulo); v. 14, n. 4, p. 534-540, 2016-12-00.
Abstract

ABSTRACT Objective To evaluate the incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with sperm retrieval by epididymal aspiration (percutaneous epididymal sperm aspiration). Methods A case-control study comprising male children of couples in which the man had been previously vasectomized and chose vasectomy reversal (n=31) or in vitro fertilization with sperm retrieval by percutaneous epididymal sperm aspiration (n=30) to conceive new children, and a Control Group of male children of fertile men who had programmed vasectomies (n=60). Y-chromosome microdeletions research was performed by polymerase chain reaction on fathers and children, evaluating 20 regions of the chromosome. Results The results showed no Y-chromosome microdeletions in any of the studied subjects. The incidence of Y-chromosome microdeletions in individuals born from vasectomized fathers who underwent vasectomy reversal or in vitro fertilization with spermatozoa recovered by percutaneous epididymal sperm aspiration did not differ between the groups, and there was no difference between control subjects born from natural pregnancies or population incidence in fertile men. Conclusion We found no association considering microdeletions in the azoospermia factor region of the Y chromosome and assisted reproduction. We also found no correlation between these Y-chromosome microdeletions and vasectomies, which suggests that the assisted reproduction techniques do not increase the incidence of Y-chromosome microdeletions. (AU)

FAPESP's process: 11/17623-5 - Incidence of Y chromosome microdeletions in children of father underwent to vasectomy reversal or in vitro fertilization with percutaneous epididimal sperm aspiration
Grantee:Sidney Glina
Support type: Regular Research Grants