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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial Potential and Cytotoxicity of Silver Nanoparticles Phytosynthesized by Pomegranate Peel Extract

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Fernandes, Renan Aparecido [1, 2] ; Berretta, Andresa Aparecida [3] ; Torres, Elina Cassia [3] ; Moreira Buszinski, Andrei Felipe [3] ; Fernandes, Gabriela Lopes [2] ; Mendes-Gouvea, Carla Correa [2] ; de Souza-Neto, Francisco Nunes [4] ; Gorup, Luiz Fernando [4, 5] ; de Camargo, Emerson Rodrigues [4] ; Barbosa, Debora Barros [2]
Total Authors: 10
[1] Univ Ctr Adamantina UNIFAI, Dept Dent, BR-17800000 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Dent Mat & Prosthodont, Sch Dent, BR-16015050 Sao Paulo - Brazil
[3] Apis Flora Ind & Comercial Ltda, Lab Res Dev & Innovat, BR-14020670 Sao Paulo - Brazil
[4] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Paulo - Brazil
[5] Fed Univ Grande Dourados, Dept Chem, FACET, BR-79804970 Dourados, MS - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ANTIBIOTICS-BASEL; v. 7, n. 3 SEP 2018.
Web of Science Citations: 4

The phytosynthesis of metal nanoparticles is nowadays attracting the increased attention of researchers and is much needed given the worldwide matter related to environmental contamination. The antimicrobial activity of colloidal and spray formulation of silver nanoparticles (AgNPs) synthesized by pomegranate peel extract against Candida albicans and Staphylococcus aureus, and their cytotoxicity in mammalian cells were tested in the present study. Dry matter, pH, total phenolics, and ellagic acid in the extract were determined. Then, AgNPs were phytosynthesized and characterized by X-ray diffraction, electron transmission microscopy, dynamic light scattering, zeta potential, and Ag+ dosage. Spray formulations and respective chemical-AgNP controls were prepared and tested. The peel extract reduced more than 99% of Ag+, and produced nanoparticles with irregular forms and an 89-nm mean size. All AgNP presented antimicrobial activity, and the spray formulation of green-AgNP increased by 255 and 4 times the effectiveness against S. aureus and C. albicans, respectively. The cytotoxicity of colloidal and spray green-AgNP was expressively lower than the respective chemical controls. Pomegranate peel extract produced stable AgNP with antimicrobial action and low cytotoxicity, stimulating its use in the biomedical field. (AU)

FAPESP's process: 16/04230-9 - Extract obtaining and synthesis of silver nanoparticles from Punica granatum (pomegranate): Physico-chemical characterization, determination of antimicrobial activity and potential healing
Grantee:Renan Aparecido Fernandes
Support Opportunities: Scholarships in Brazil - Doctorate