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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanosynthesis of Silver-Calcium Glycerophosphate: Promising Association against Oral Pathogens

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Fernandes, Gabriela Lopes [1] ; Botazzo Delbem, Alberto Carlos [2] ; do Amaral, Jackeline Gallo [2] ; Gorup, Luiz Fernando [3, 4] ; Fernandes, Renan Aparecido [5, 1] ; de Souza Neto, Francisco Nunes [3] ; Santos Souza, Jose Antonio [2] ; Monteiro, Douglas Roberto [6] ; Agostinho Hunt, Alessandra Marcal [7] ; Camargo, Emerson Rodrigues [3] ; Barbosa, Debora Barros [1]
Total Authors: 11
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, BR-16015050 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Pediat Dent & Publ Hlth, BR-16015050 Sao Paulo - Brazil
[3] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Paulo - Brazil
[4] Fed Univ Grande Dourados, Dept Chem, FACET, BR-79804970 Dourados, MS - Brazil
[5] Univ Ctr Adamantina UNIFAI, Dept Dent, BR-17800000 Sao Paulo - Brazil
[6] Univ Western Sao Paulo UNOESTE, Grad Program Dent, BR-19050920 Sao Paulo - Brazil
[7] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48823 - USA
Total Affiliations: 7
Document type: Journal article
Source: ANTIBIOTICS-BASEL; v. 7, n. 3 SEP 2018.
Web of Science Citations: 3

Nanobiomaterials combining remineralization and antimicrobial abilities would bring important benefits to control dental caries. This study aimed to produce nanocompounds containing calcium glycerophosphate (CaGP) and silver nanoparticles (AgNP) by varying the reducing agent of silver nitrate (sodium borohydride (B) or sodium citrate (C)), the concentration of silver (1% or 10%), and the CaGP forms (nano or commercial), and analyze its characterization and antimicrobial activity against ATCC Candida albicans (10231) and Streptococcus mutans (25175) by the microdilution method. Controls of AgNP were produced and silver ions (Ag+) were quantified in all of the samples. X-ray diffraction, UV-Vis, and scanning electron microscopy (SEM) analysis demonstrated AgNP associated with CaGP. Ag+ ions were considerably higher in AgCaGP/C. C. albicans was susceptible to nanocompounds produced with both reducing agents, regardless of Ag concentration and CaGP form, being Ag10%CaGP-N/C the most effective compound (19.5-39.0 mu g Ag mL(-1)). While for S. mutans, the effectiveness was observed only for AgCaGP reduced by citrate, also presenting Ag10%CaGP-N the highest effectiveness (156.2-312.5 mu g Ag mL(-1)). Notably, CaGP enhanced the silver antimicrobial potential in about two- and eight-fold against C. albicans and S. mutans when compared with the AgNP controls (from 7.8 to 3.9 and from 250 to 31.2 mu g Ag mL(-1), respectively). The synthesis that was used in this study promoted the formation of AgNP associated with CaGP, and although the use of sodium borohydride (B) resulted in a pronounced reduction of Ag+, the composite AgCaGP/B was less effective against the microorganisms that were tested. (AU)

FAPESP's process: 14/08648-2 - Ag/²-calcium glycerophosphate nanocomposite: synthesis, and effect on Candida albicans and Streptococcus mutans biofilm and on odontoblasts MDPC-23 cells
Grantee:Gabriela Lopes Fernandes
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/24200-9 - Effect of nanocomposite Ag/B-calcium glycerophosphate on Candida albicans and Streptococcus mutans biofilm and on human pulp dental cells in the presence and absence of these biofilms
Grantee:Debora Barros Barbosa
Support type: Regular Research Grants