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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization and in vitro and in vivo assessment of poly(butylene adipate-co-terephthalate)/nano-hydroxyapatite composites as scaffolds for bone tissue engineering

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Silva, Aline dos Santos [1] ; Manzolli Rodrigues, Bruno Vinicius [2] ; Oliveira, Francilio Carvalho [2, 3] ; Carvalho, Jancineide Oliveira [2, 3] ; Reis de Vasconcellos, Luana Marotta [4] ; Ribeiro de Araujo, Juliani Caroline [4] ; Marciano, Fernanda Roberta [2] ; Lobo, Anderson Oliveira [2, 5]
Total Authors: 8
[1] Univ Porto, Fac Engn FEUP, P-4200465 Porto - Portugal
[2] Univ Brasil, Inst Cient & Tecnol, BR-08230030 Sao Paulo, SP - Brazil
[3] Ctr Univ UNINOVAFAPI, BR-64073505 Teresina, Piaui - Brazil
[4] Univ Estadual Paulista, Inst Ciencia & Tecnol, BR-12245000 Sao Jose Dos Campos, SP - Brazil
[5] Univ Fed Piaui, Lab Interdisciplinhar Mat Avancados, Programa Posgrad Ciencia & Engn Mat, Campus Univ Ministro Petronio Portella Bairro, BR-64049550 Teresina, Piaui - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF POLYMER RESEARCH; v. 26, n. 2 FEB 1 2019.
Web of Science Citations: 1

The polyester for bone tissue engineering is produced using different concentrations of hydroxyapatite. Poly(butylene adipate-co-terephthalate) (PBAT) solutions containing different concentrations of nano-hydroxyapatite (nHAp) (1, 2, 3, 4, 5 and 6% wt) were evaluated to assess their potential to produce scaffolds via electrospinning. The characteristics of these solutions were evaluated using surface tension analysis. Different solutions were electrospun and characterized using scanning electron microscopy, X-ray diffractometry (XRD), infrared spectroscopy (FTIR), and differential scanning calorimetry analysis. In vitro and in vivo experiments were performed using MG-63 osteoblast cells and male Wistar rats. The MTT assay was used to evaluate the cytotoxicity of scaffolds. Microtomography and a three-point bend test were used to analyze parameters of bone neoformation 4 weeks after implantation. We observed a requirement for solutions containing nHAp to reach chemical stability to produce ultrathin fibers. FTIR and XRD data demonstrated the presence of carbonate and phosphate groups, and thermal analysis showed a reduction in crystallinity of the nanocomposites when the concentration of nHAp was increased. None of the scaffolds analyzed demonstrated cytotoxicity when compared to controls. All of the PBAT/nHAp scaffolds analyzed promoted bone repair; however, a solution of PBAT with 3% nHAp improved bone volume, force, and stiffness when compared to controls. (AU)

FAPESP's process: 15/01259-3 - Electrospinning by solution of PBAT built nanohidroxiapatita
Grantee:Aline dos Santos Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/04618-7 - Influence of scaffolds of poly(butylene adipate-co-terephthalate) embedded with carbon and nano-hydroxyapatite nanotubes in bone repair of critical defects
Grantee:Juliani Caroline Ribeiro de Araújo
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants