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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene Deletion Leads to Improved Valinomycin Production by Streptomyces sp. CBMAI 2042

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Paulo, Bruno S. [1] ; Sigrist, Renata [1] ; Angolini, Celio F. F. [2] ; Eberlin, Marcos N. [3] ; de Oliveira, Luciana G. [1]
Total Authors: 5
[1] Univ Estadual Campinas, Inst Quim, CP 6154, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed ABC, Ctr Ciencias Nat & Humans, BR-09210580 Santo Andre, SP - Brazil
[3] Univ Presbiteriana Mackenzie, BR-01301100 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 30, n. 3, SI, p. 673-679, MAR 2019.
Web of Science Citations: 2

The genus Streptomyces represents one of the largest producers of molecules with antibiotic activity. The whole genome sequencing of the endophytic microorganism Streptomyces sp. CBMAI 2042 revealed 35 gene clusters encoding for secondary metabolism including 3 non-ribosomal peptide synthetases (NRPS) and 7 NRPS-hybrids. Combining genome mining and cultivation profile analysis, the depsipeptide ionophore valinomycin was identified as one of the main metabolites produced by this strain. To better understand the metabolic machinery codified in CBMAI 2042 genome an adenylation domain from a hybrid NRPS cluster was deleted through a double crossing knockout experiment. Though the deletion was not plentiful to elucidate the encoded NRP metabolite related to the adenilation domain, an astounding increase of 10.5-fold in valinomycin production was observed in the mutant. These results suggest a metabolic flux redistribution of common substrates as an outcome of a gene target deletion. (AU)

FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Grantee:Arlene Gonçalves Corrêa
Support Opportunities: Research Grants - Research Centers in Engineering Program
FAPESP's process: 15/01013-4 - Mining a t1PKS-transAT PKS-NRPS in Streptomyces sp. genome
Grantee:Renata Sigrist
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 10/51677-2 - Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine
Grantee:Marcos Nogueira Eberlin
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/12727-5 - Genome mining in Streptomyces
Grantee:Luciana Gonzaga de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/12598-8 - Mining a of t1PKS-transAT PKS-NRPS in Streptomyces sp.
Grantee:Renata Sigrist
Support Opportunities: Scholarships in Brazil - Master