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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Novel pseudo-aspartic peptidase from the midgut of the tick Rhipicephalus microplus

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Lu, S. [1] ; Parizi, L. F. [2] ; Torquato, R. J. S. [1] ; Vaz Junior, I. S. [2, 3, 4] ; Tanaka, A. S. [1, 3]
Total Authors: 5
[1] Fed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil
[2] Fed Univ Rio Grande do Sul UFRGS, Ctr Biotechnol, Porto Alegre, RS - Brazil
[3] Natl Inst Sci & Technol Mol Entomol INTC EM, Porto Alegre, RS - Brazil
[4] Fed Univ Rio Grande do Sul UFRGS, Sch Vet, Porto Alegre, RS - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, JAN 24 2019.
Web of Science Citations: 0

The characterization of Rhipicephalus microplus tick physiology can support efforts to develop and improve the efficiency of control methods. A sequence containing a domain with similarity to one derived from the aspartic peptidase family was isolated from the midgut of engorged female R. microplus. The lack of the second catalytic aspartic acid residue suggest that it may be a pseudo-aspartic peptidase, and it was named RmPAP. In this work we confirm the lack of proteolytic activity of RmPAP and investigate it's non-proteolytic interaction with bovine hemoglobin by Surface Plasmon Resonance and phage display. Moreover we carried out RNAi interference and artificial feeding of ticks with anti-RmPAP antibodies to assess it's possible biological role, although no changes were observed in the biological parameters evaluated. Overall, we hypothesize that RmPAP may act as a carrier of hemoglobin/heme between the tick midgut and the ovaries. (AU)

FAPESP's process: 15/09268-1 - Characterization of two proteases from Babesia bovis parasite: Importance in the host infections.
Grantee:Stephen Lu
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/03657-8 - Inhibitor and proteases of ectoparasites: relationship of structure-function and identification of the role of these molecules in the interaction of diseases vector e their etiological agents
Grantee:Aparecida Sadae Tanaka
Support type: Research Projects - Thematic Grants