Fed Univ Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil
 Fed Univ Rio Grande do Sul UFRGS, Ctr Biotechnol, Porto Alegre, RS - Brazil
 Natl Inst Sci & Technol Mol Entomol INTC EM, Porto Alegre, RS - Brazil
 Fed Univ Rio Grande do Sul UFRGS, Sch Vet, Porto Alegre, RS - Brazil
Total Affiliations: 4
JAN 24 2019.
Web of Science Citations:
The characterization of Rhipicephalus microplus tick physiology can support efforts to develop and improve the efficiency of control methods. A sequence containing a domain with similarity to one derived from the aspartic peptidase family was isolated from the midgut of engorged female R. microplus. The lack of the second catalytic aspartic acid residue suggest that it may be a pseudo-aspartic peptidase, and it was named RmPAP. In this work we confirm the lack of proteolytic activity of RmPAP and investigate it's non-proteolytic interaction with bovine hemoglobin by Surface Plasmon Resonance and phage display. Moreover we carried out RNAi interference and artificial feeding of ticks with anti-RmPAP antibodies to assess it's possible biological role, although no changes were observed in the biological parameters evaluated. Overall, we hypothesize that RmPAP may act as a carrier of hemoglobin/heme between the tick midgut and the ovaries. (AU)