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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Behavioral and neurochemical characterization of the mlh mutant mice lacking otoconia

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Manes, Marianna [1] ; Aranha Garcia-Gomes, Mariana de Souza [1] ; Sandini, Thaisa Meira [2] ; Zaccarelli-Magalhaes, Julia [1] ; Florio, Jorge Camilo [1] ; Alexandre-Ribeiro, Sandra Regina [3] ; Wadt, Danilo [1] ; Bernardi, Maria Martha [4] ; Gomes Massironi, Silvia Maria [3, 1] ; Cabrera Mori, Claudia Madalena [1]
Total Authors: 10
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil
[4] Paulista Univ UNIP, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Behavioural Brain Research; v. 359, p. 958-966, FEB 1 2019.
Web of Science Citations: 4

Otoconia are crucial for the correct processing of positional information and orientation. Mice lacking otoconia cannot sense the direction of the gravity vector and cannot swim properly. This study aims to characterize the behavior of mergulhador (mlh), otoconia-deficient mutant mice. Additionally, the central catecholamine levels were evaluated to investigate possible correlations between behaviors and central neurotransmitters. A sequence of behavioral tests was used to evaluate the parameters related to the general activity, sensory nervous system, psychomotor system, and autonomous nervous system, in addition to measuring the acquisition of spatial and declarative memory, anxiety-like behavior, motor coordination, and swimming behavior of the mlh mutant mice. As well, the neurotransmitter levels in the cerebellum, striatum, frontal cortex, and hippocampus were measured. Relative to BALB/c mice, the mutant mlh mice showed 1) reduced locomotor and rearing behavior, increased auricular and touch reflexes, decreased motor coordination and increased micturition; 2) decreased responses in the T-maze and aversive wooden beam tests; 3) increased time of immobility in the tail suspension test; 4) no effects in the elevated plus maze or object recognition test; 5) an inability to swim; and 6) reduced turnover of dopaminergic system in the cerebellum, striatum, and frontal cortex. Thus, in our mlh mutant mice, otoconia deficiency reduced the motor, sensory and spatial learning behaviors likely by impairing balance. We did not rule out the role of the dopaminergic system in all behavioral deficits of the mlh mutant mice. (AU)

FAPESP's process: 16/03180-8 - Phenotypic characterization and central nervous system analysis of mutant mice equilíbrio and mergulhador induced with the chemical mutagen ethylnitrosourea
Grantee:Marianna Manes
Support Opportunities: Scholarships in Brazil - Master