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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Capillary electrophoresis with dual diode array detection and tandem mass spectrometry to access cardiovascular biomarkers candidates in human urine: Trimethylamine-N-Oxide and L-carnitine

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Cieslarova, Zuzana [1] ; Magaldi, Mariana [2] ; Barros, Lucelia Alcantara [3] ; do Lago, Claudimir Lucio [1] ; Oliveira, Daniel Rossado [1] ; Helfenstein Fonseca, Francisco Antonio [4] ; Izar, Maria Cristina [4] ; Lopes, Aline Soriano [3] ; Maggi Tavares, Marina Franco [1] ; Klassen, Aline [3]
Total Authors: 10
[1] Univ Sao Paulo, Inst Chem, Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Pharmaceut Sci, Diadema, SP - Brazil
[3] Fed Univ Sao Paulo UNIFESP, Dept Chem, Rua Prof Arthur Riedel 275, BR-09972270 Diadema, SP - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Dept Med, Div Cardiol, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Chromatography A; v. 1583, p. 136-142, JAN 4 2019.
Web of Science Citations: 4

A capillary electrophoresis with diode array and tandem mass spectrometry detection (CE-UV-MS/MS) method has been developed for the targeted assessment of cardiovascular biomarkers candidates, trimethylamine-N-Oxide (TMAO) and L-carnitine, and creatinine in human urine samples. The dual detection was applied due to the high concentration of creatinine (monitored by UV detection at 200 nm) in relation to TMAO and t-carnitine (quantified by selected reaction monitoring (SRM) mass spectrometry), in human urine. All instrumental parameters, sheath liquid (SHL) and background electrolyte (BGE) compositions were optimized with a pool of urine provided by adult healthy volunteers and evaluated by signal-to-noise ratio (SNR) and peak shape of TMAO. The compositions for the optimized BGE was formic acid at concentration of 0.10 mol L-1, and for SHL was 70:30 MeOH:H2O containing 0.05% (v/v) formic acid, delivered at a flow rate of 5 mu L min(-1). Limits of detection for TMAO, L-carnitine and creatinine were 0.76, 0.54 and 303 mu mol L-1, respectively. Limits of quantification were 2.5, 1.8 and 1000 mu mol L-1, respectively. Linearity was evaluated by ANOVA and presented R-2 from 0.993 to 0.997. Precision and accuracy were evaluated at three concentration levels. Coefficients of variation (CV) from 1 to 21% were obtained for the intra-day precision evaluation and from 2 to 16% for the inter-day precision evaluation. The recovery ranged from 75 to 116%. Quantitation of TMAO and L-carnitine in infarcted patients urine in comparison to healthy individuals indicated a 2.2 fold increase of TMAO and a 7.0 fold increase of L-carnitine. These results showed the potential applicability of the proposed method for the evaluation of TMAO and L-carnitine in urine within a panel of candidate metabolites in targeted metabolomics studies of cardiovascular diseases among other conditions. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/51692-7 - Role of adaptative immunity on the progress of ischemic heart disease after acute myocardial infarction
Grantee:Francisco Antonio Helfenstein Fonseca
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/06642-1 - Formation and properties of adducts of organic hydroxy-compounds with carbonate in aqueous medium and biological conditions
Grantee:Claudimir Lucio Do Lago
Support Opportunities: Regular Research Grants
FAPESP's process: 17/27059-6 - Expanding the metabolic coverage using ion chromatography-mass spectrometry in metabolomics studies of immunosuppressive therapies in renal transplantation
Grantee:Marina Franco Maggi Tavares
Support Opportunities: Regular Research Grants
FAPESP's process: 16/19045-2 - Determination of adenosine and differential protein after acute myocardial infartion
Grantee:Lucelia Alcantara Barros
Support Opportunities: Scholarships in Brazil - Scientific Initiation