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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antioxidant role on the protection of melanocytes against visible light-induced photodamage

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Freitas, Juliana Vescovi [1] ; Junqueira, Helena Couto [2] ; Martins, Waleska Kerllen [2, 3] ; Baptista, Mauricio S. [2] ; Gaspar, Lorena Rigo [1]
Total Authors: 5
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[3] Univ Anhanguera Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Free Radical Biology and Medicine; v. 131, p. 399-407, FEB 1 2019.
Web of Science Citations: 2

Visible light can induce the generation of singlet oxygen and can cause oxidative stress, especially in melanocytes due to melanin photosensitization. Currently, there is no organic UV-filter that provide visible light protection. Previous studies showed that some antioxidants, such as apigenin (API), chrysin (CRI) and beta-carotene (BTC) besides neutralizing radical chain reactions can also quench singlet oxygen via physical or chemical quenching and exhibit potential for use in photoprotection. Therefore, the aim of this study is to evaluate the efficacy of API, CRI and BTC on the protection against cell death induced by melanin photosensitization and understand the underlying mechanisms that are involved in the protection. Precise protocols of melanogenesis and quantification of singlet oxygen generation were developed. Viability of B16-F10 cells with melanin basal levels and after melanogenesis induction was evaluated after visible light exposure in the presence and absence of API, CRI and BTC. Results showed that API and BTC protected cells from photoinduced cell death API exhibiting superior photoprotective effect. We noticed that the efficiency of cell protection and the rate of singlet oxygen suppression are not well correlated, at least for the studied series of antioxidants, indicating that the anti-radical capacity should be playing a major role in protecting cells against the damage induced by melanin photosensitization. In terms of sun care strategies, both API and BTC offer protection against visible light-induced damages and may be effective topical antioxidants to be added to sunscreens. (AU)

FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/50680-5 - Photosensitization in life sciences
Grantee:Mauricio da Silva Baptista
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/07642-6 - Autophagy activation/inhibition by triterpenoids and the impact of membranes interaction: therapeutic implications on tumor response
Grantee:Waleska Kerllen Martins Gardesani
Support Opportunities: Regular Research Grants