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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sporothrix schenckii CellWall Proteins-Stimulated BMDCs Are Able to Induce a Th1-Prone Cytokine Profile In Vitro

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Quinello, Camila [1] ; Ferreira, Lucas Souza [1] ; Picolli, Isabella [1] ; Loesch, Maria Luiza [1] ; Portuondo, Deivys Leandro [1] ; Batista-Duharte, Alexander [1] ; Carlos, Iracilda Zeppone [1]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Rodovia Araraquara Jau Km 1, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF FUNGI; v. 4, n. 3 SEP 2018.
Web of Science Citations: 3

Sporotrichosis is a subcutaneous mycosis affecting humans and other animals. The disease can be acquired by accidental inoculation of the fungus through the skin or through the respiratory system. Sporotrichosis can also be transmitted through bites or scratches by infected cats and more rarely by other animals (zoonotic transmission). Conventional antifungal therapy is especially inefficient in immunocompromised patients, who tend to develop the most severe forms of the disease, thus prompting the search for alternative therapies. Given their antigen-presenting properties, dendritic cells (DCs) have been used in both prophylactic and therapeutic vaccination strategies. Hence, this study aims to assess the use of DCs as a prophylactic tool in sporotrichosis by evaluating the immune profile induced by Sporothrix schenckii cell wall proteins (SsCWP)-stimulated, bone-marrow-derived DCs (BMDCs). Mouse BMDCs were stimulated with SsCWP for 24 h and analyzed for the surface expression of costimulatory molecules and TLR-4, as well as for the secretion of proinflammatory cytokines and IL-10. Following that, activated BMDCs were cocultured with splenocytes for 72 h and had the same cytokines measured in the supernatant. SsCWP-stimulated BMDCs showed higher expression of CD80, CD86, and CD40, but not TLR-4, and higher secretion of IL-6, IL-17A, and TNF. On the other hand, higher levels of IFN-gamma, IL-10, and IL-2 were found in the supernatants of the coculture as compared with the BMDCs alone; TNF secretion was almost completely abrogated, whereas IL-6 was only partially inhibited and IL-17A was unaffected. Our results thus suggest that SsCWP-stimulated BMDCs are able to induce a Th1-prone cytokine profile which is known to be protective against other fungal diseases. This result could lead to studies which evaluate the development of prophylactic and/or therapeutic DC-based tools against sporotrichosis. (AU)

FAPESP's process: 15/04023-0 - Evaluation of activated isogenic dendritic cells and imiquimod as therapeutic alternative in sporotrichosis on immunosuppressed and immunocompetent animals
Grantee:Iracilda Zeppone Carlos
Support Opportunities: Regular Research Grants
FAPESP's process: 14/00914-5 - Vaccination with activated dendritic cells in the prophylaxis of experimental sporotrichosis in immunosuppressed mice
Grantee:Camila Cristina Quinello Gomes de Faria
Support Opportunities: Scholarships in Brazil - Post-Doctoral