Prepubertal exposure to low doses of sodium arseni... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prepubertal exposure to low doses of sodium arsenite impairs spermatogenesis and epididymal histophysiology in rats

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Author(s):
de Medeiros, Paloma da Cunha [1] ; Samelo, Ricardo Rodrigues [1] ; Gomes Silva, Ana Priscila [1] ; Araujo Santiago, Marcella da Silva [1] ; Duarte, Fabio Andrei [2] ; de Castro, Italo Braga [3] ; Perobelli, Juliana Elaine [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Mar, Lab Toxicol Expt LATOEX, Campus Baixada Santista, Santos, SP - Brazil
[2] Univ Fed Santa Maria, Dept Quim, Santa Maria, RS - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Mar, Campus Baixada Santista, Santos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ENVIRONMENTAL TOXICOLOGY; v. 34, n. 1, p. 83-91, JAN 2019.
Web of Science Citations: 6
Abstract

For the first time, juvenile toxicity of inorganic arsenic (As) was investigated in male rats, focusing on reproductive effects. As is a metalloid naturally occurring in the environment, being the inorganic forms the most toxics. Contaminated drinking water and agricultural products are the main prospectors of intoxication for general population. In the present study, Wistar male rats (21 days old) were distributed into three groups (n = 10/group): control (received vehicle-filtered drinking water), As1 (received AsNaO2 at 0.01 mg L-1) and As2 (received AsNaO2 at 10 mg L-1). The animals were euthanized on PND 53. Testicular damages increased in As1 and As2 compared to control (ie, presence of vacuolization, acidophilic cells, and epithelium degeneration). Testicular interstitium of As1 and As2 presented fluid's increase and intense inflammatory infiltration. In the epididymis there was reduction of sperm amount in the lumen, besides epithelium areas presenting cribriform aspect in As1 and As2, exfoliation of cells in the light (in As1) and vacuoles (in As2). In epididymis interstitium, inflammatory infiltrates were observed in initial segment of As1 and As2. AsNaO2 changed immunolabeling pattern for androgen receptor in epididymis of As2, although serum testosterone levels was statistically comparable to control. Mass spectrometry revealed higher As concentrations in testis and epididymis of As2 compared to As1 and Control. These results indicate compromise of spermatogenesis and epididymal histophysiology in AsNaO2-treated animals, possibly impairing sperm quality and fertility in long-term, even at low levels of exposure. Investigations about the reversibility of reproductive damages are necessary to better understand the mechanisms of As reproductive toxicity. (AU)

FAPESP's process: 15/25821-2 - Evaluation of reproductive parameters of male rats exposed to sodium arsenite: a juvenile toxicity study
Grantee:Paloma da Cunha de Medeiros
Support Opportunities: Scholarships in Brazil - Scientific Initiation