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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients

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Cacador, Natalia Candido [1] ; da Costa Capizzani, Carolina Paulino [1] ; Monteiro Marin Torres, Lfdia Alice Gomes [2] ; Galetti, Renata [1] ; Ciofu, Oana [3] ; da Costa Darini, Ana Lticia [1] ; Flefiby, Niels [3, 4]
Total Authors: 7
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pediat, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[3] Univ Copenhagen, Dept Immunol & Microbiol, Copenhagen - Denmark
[4] Univ Hosp, Rigshosp, Dept Clin Microbiol, Copenhagen - Denmark
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 13, n. 11 NOV 29 2018.
Web of Science Citations: 3

Chronic lung infection by Pseudomonas aeruginosa is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. This is associated with the conversion of the non-mucoid to the mucoid phenotype. However, there is little information about the occurrence of alginate-producing P. aeruginosa in CF patients outside Europe and North America. The aim of the present study was to investigate mutations in the algTmucABD operon in mucoid and non-mucoid isolates from Brazilian CF patients. Twenty-seven mucoid and 37 non-mucoid isolates from 40 CF patients chronically infected by P. aeruginosa attending a CF reference center in Brazil were evaluated by sequence analysis. Mutations in mucA were observed in 93% of the mucoid isolates and 54% of the non-mucoid isolates. Among these non-mucoid isolates, 55% were considered revertants, since they also had mutations in algT (algU). Most isolates associated with moderate alginate production presented point mutations in mucB and/or mucD. We identified 30 mutations not previously described in the operon. In conclusion, mutations in mucA were the main mechanism of conversion to mucoidy, and most of the non-mucoid isolates were revertants, but the mechanism of revertance is not fully explained by changes in algT. (AU)

FAPESP's process: 13/13455-6 - Epidemiology of bacterial infections in patients with cystic fibrosis involving Achromobacter and Burkholderia cepacia complex bacteria
Grantee:Carolina Paulino da Costa Capizzani
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/11728-0 - Complete sequencing of plasmids carrying antibiotic resistance genes in bacteria of interest and genome analysis of Pseudomonas aeruginosa-producing SPM-1 and KPC-2 lines by large-scale sequencing
Grantee:Renata Galetti
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
Grantee:Ana Lúcia da Costa Darini
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/13358-0 - Classical and molecular epidemiology of Pseudomonas aeruginosa isolated from patients with Cystic Fibrosis
Grantee:Natália Candido Caçador
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)