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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples

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Souza, Israel D. [1] ; Hantao, Leandro W. [2] ; Queiroz, Maria Eugenia C. [1]
Total Authors: 3
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Analytica Chimica Acta; v. 1045, p. 108-116, JAN 3 2019.
Web of Science Citations: 9

This manuscript describes the development of wall-coated open tubular capillary column with polymeric ionic liquids (PILs) for on-line in-tube solid phase microextraction coupled with ultra high-performance liquid chromatography tandem mass spectrometry (in-tube SPME/UHPLC-MS/MS) to determine anandamide (AEA) and 2-arachidonoyl glycerol (2 A G) in plasma samples. Selective PILs were synthetized from the {[}VC6IM]{[}Cl], {[}VC16IM]{[}Br], and {[}(VIM)(2)C-10]2 {[}Br] - ionic liquids -by in-situ thermal-initiated polymerization in a fused silica capillary column for in-tube SPME. The synthesis procedure was optimized, and the capillary columns were characterized using spectroscopic and chromatography techniques. The chemically bonded and cross-linked PIL-based sorbent phase (thickness coating: 1.7 mu m) presented high chemical and mechanical stability. Among the sorbents evaluated, the PIL-based capillary, {[}VC16IM]{[}Br]/{[}(VIM)(2)C-10]2 {[}Br] presented the best performance with a sorption capacity of 37,311 ng cm(-3) and 48,307 ng cm(-3) for AEA and 2 A G, respectively. This capillary was reused more than ninety times without significant changes in extraction efficiency. The in-tube SPME-UHPLC-MS/MS method presented a linear range from 0.1 ngmL(-1) to 100 ngmL(-1) for AEA, and from 0.05 ng mL(-1) to 100 ngmL(-1) for 2 A G, with coefficients of determination higher than 0.99, p-value for Lack-of-fit test higher than 0.05 (alpha of 0.05), precision with coefficient of variation (CV) values ranging from 1.6 to 14.0% and accuracy with relative standard deviation (RSD) values from -19.6% to 13.2%. This method was successfully applied to determine AEA and 2 A G in plasma patients with Parkinson's disease. The concentrations in these plasma samples ranged from 0.14 to 0.46 ngmL(-1) for AEA and from <0.05 ng mL(-1) to 0.51 ng mL(-1) for 2-AG. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
Grantee:Maria Eugênia Queiroz Nassur
Support type: Regular Research Grants
FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
Grantee:Fernando Mauro Lanças
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/01082-9 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids in plasma samples
Grantee:Israel Donizeti de Souza
Support type: Scholarships in Brazil - Doctorate