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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases

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Marchioni, Camila [1] ; de Souza, Israel Donizeti [2] ; Acquaro Junior, Vinicius Ricardo [2] ; de Souza Crippa, Jose Alexandre [3] ; Tumas, Vitor [3] ; Costa Queiroz, Maria Eugenia [1, 2]
Total Authors: 6
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Review article
Source: Analytica Chimica Acta; v. 1044, p. 12-28, DEC 31 2018.
Web of Science Citations: 12

Endocannabinoids (ECs) are endogenous lipid-based retrograde neurotransmitters that bind to cannabinoid receptors {[}cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2)]. Many ECs have been characterized; anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) are still considered the primary ECs signaling mediators. Dysregulation of ECs has been implicated in a wide range of pathologies, including neurodegenerative diseases. Understanding how ECs participate in neurological diseases is important to describe the pathology and to establish new treatments. Considering the physicochemical properties of ECs, liquid chromatography coupled to tandem mass spectrometry has become the reference method to determine these endogenous substances, in trace levels, in different biological samples. This review describes the recent advances in LC-MS/MS methods designed to determine ECs in complex biological matrixes. The advantages, limitations, selectivity, matrix effect, and sensitivity associated with each approach are emphasized. This article comprises three sections: (I) sample preparation techniques (conventional, microextraction, and online systems), (II) chromatographic methods (especially LC-MS/MS), and (III) relationship between ECs levels and neurodegenerative diseases. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
Grantee:Fernando Mauro Lanças
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
Grantee:Maria Eugênia Queiroz Nassur
Support type: Regular Research Grants
FAPESP's process: 16/13639-8 - Column-switching liquid chromatography-Tandem mass spectrometry for determination of endocannabinoids and cannabinoids in biological samples from patients with Parkinson's Disease
Grantee:Camila Marchioni
Support type: Scholarships in Brazil - Doctorate (Direct)