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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

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Leal, D. F. [1] ; Torres, M. A. [1] ; Ravagnani, G. M. [1] ; Martins, S. M. M. K. [1] ; Meirelles, F. V. [2] ; de Andrade, A. F. C. [1]
Total Authors: 6
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Swine Res Ctr, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Animal Reproduction Science; v. 198, p. 20-26, NOV 2018.
Web of Science Citations: 1

Seminal plasma (SP), the fluid that surrounds the sperm cells, is known to exert substantial influence on sperm physiology. The SP has a pivotal role in sperm function in vivo, and due to its components, it functions in an ambiguous manner in vitro, simultaneously possessing deleterious and beneficial effects. This experiment aimed to describe the differences between the presence or absence of SP from the sperm-rich fraction on some spermatozoa characteristics (kinetics, plasma and acrosome membrane integrity, lipid peroxidation and capacitation-like changes). Furthermore, this experiment focused on distinguishing the effects of SP on the variables evaluated from the effects of centrifugation during SP removal. Total and progressive sperm motility, as well as integrity of plasma and acrosome membranes, were less (P < 0.05) in the absence of SP. Membrane lipid peroxidation (P < 0.05) and sperm membrane stability (P < 0.05) did not differ among treatments. The SP from the sperm-rich fraction is important for the maintenance of adequate structural and functional characteristics of extended liquid boar semen and should be present in seminal doses throughout storage. Furthermore, the detrimental effect on the variables evaluated was caused solely by the absence of SP and not by the process of removal through centrifugation at 500 x g for 10 min. (AU)

FAPESP's process: 14/20768-3 - Influence of seminal plasma derived from the rich fraction of the ejaculate on the structural characteristics and the kinetics of the swine sperm stored under refrigeration at 17°C for 72 hours
Grantee:Diego Feitosa Leal
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/14258-5 - Influence of seminal plasma from the rich fraction of boar ejaculate on structural characteristics, kinetics, capacitation and fertility of the swine spermatozoa stored at 17°C for 72 hours
Grantee:André Furugen Cesar de Andrade
Support Opportunities: Regular Research Grants