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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunological and microbiological periodontal profiles in isolated growth hormone deficiency

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Araujo, I. M. P. [1] ; Albuquerque-Souza, E. [1] ; Aguiar-Oliveira, M. H. [2] ; Holzhausen, M. [1] ; Oliveira-Neto, L. A. [2] ; Salvatori, R. [3] ; Saraiva, L. [1] ; Mayer, M. P. A. [4] ; Pannuti, C. M. [1] ; Ribeiro, A. O. [5, 6] ; Romito, G. A. [1] ; Pustiglioni, F. E. [1]
Total Authors: 12
[1] Univ Sao Paulo, Sch Dent, Div Periodont, Sao Paulo - Brazil
[2] Univ Fed Sergipe, Div Endocrinol, BR-49060100 Aracaju, SE - Brazil
[3] Johns Hopkins Univ, Sch Med, Dept Med, Div Endocrinol, Baltimore, MD - USA
[4] Univ Sao Paul, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[5] Univ Fed Sergipe, Div Immunol, Aracaju, SE - Brazil
[6] Univ Fed Sergipe, Mol Biol Lab, Aracaju, SE - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Journal of Periodontology; v. 89, n. 11, p. 1351-1361, NOV 2018.
Web of Science Citations: 1

Background: Growth hormone (GH) has been identified as an important regulator of the immune response. We have previously shown that adults with isolated GH deficiency (IGHD) due to a mutation in the GH releasing hormone receptor (GHRHR) gene, have a greater chance of having periodontitis. However, the interaction of GH with periodontal tissues is still unknown, and this population has emerged as a unique model to investigate this issue. Therefore, we evaluated the microbiological and immunological periodontal profiles of such individuals. Methods: Nineteen IGHD and 19 controls matched by age, sex, diabetes, and smoking status, were enrolled in this case-control study. Periodontal clinical parameters (probing depth {[}PD] and clinical attachment loss {[}AL]) were measured at six sites per tooth. Immune mediators (C-reactive protein, matrix metalloproteinase {[}MMP]-8, MMP-9, interleukin {[}IL]-1 alpha, IL-6, IL-8, tumor necrosis factor {[}TNF]-alpha, adiponectin, and leptin) were analyzed by enzyme-linked immunosorbent assay (ELISA) in the gingival crevicular fluid (GCF) in four non-adjacent sites for each participant (two with PD <= 3 mm {[}shallow sites] and two with PD >= 7 mm or the worst PD found in the mouth {[}deep sites]). Bacterial quantification (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) of subgingival biofilm samples collected from these same sites was performed by quantitative real-time polymerase chain reaction (qPCR). Results: IGHD individuals presented higher values of PD and AL, and increased levels of CRP, IL-8, MMP-8, and adiponectin in the GCF. Bacterial quantification did not identify differences between the two groups. Conclusion: IGHD alters the local immune response in periodontal pockets leading to greater attachment loss, and GH stands out as an important hormone to be evaluated in the pathogenesis of periodontitis. (AU)

FAPESP's process: 11/24005-6 - Periodontal disease in adults with isolated growth hormone deficiency: clinical, microbiological and immunological aspects.
Grantee:Francisco Emílio Pustiglioni
Support Opportunities: Regular Research Grants
FAPESP's process: 15/07396-2 - Effect of protease activated receptor 1 (PAR1) activation on osteogenic activity of periodontal ligament cells
Grantee:Marinella Holzhausen Caldeira
Support Opportunities: Regular Research Grants