Campinas Univ UNICAMP, Fac Chem Engn, BR-13083970 Campinas, SP - Brazil
 Univ Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, Polo Ciencias Saude Azinhaga Santa Comba, P-3000548 Coimbra - Portugal
 Univ Coimbra, Fac Pharm, Grp Pharmaceut Technol, REQUIMTE LAQV, Coimbra - Portugal
Total Affiliations: 6
COLLOIDS AND SURFACES B-BIOINTERFACES;
NOV 1 2018.
Web of Science Citations:
The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations. (AU)