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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Solid lipid nanoparticles optimized by 2(2) factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts

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Author(s):
Rigon, Roberta Balansin [1] ; Goncalez, Maira Lima [1] ; Severino, Patricia [2] ; Alves, Danilo Antonini [3] ; Santana, Maria H. A. [4] ; Souto, Eliana B. [5, 6] ; Chorilli, Marius [1]
Total Authors: 7
Affiliation:
[1] UNESP Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Farmacos & Medicamentos, Campus Araraquara, BR-14800850 Araraquara, SP - Brazil
[2] Univ Tiradentes, Ctr Biol Sci & Hlth, BR-49010390 Aracaju, Sergipe - Brazil
[3] Campinas Univ UNICAMP, Inst Biol, Lab Biotechnol, BR-13083862 Campinas, SP - Brazil
[4] Campinas Univ UNICAMP, Fac Chem Engn, BR-13083970 Campinas, SP - Brazil
[5] Univ Coimbra FFUC, Fac Pharm, Dept Pharmaceut Technol, Polo Ciencias Saude Azinhaga Santa Comba, P-3000548 Coimbra - Portugal
[6] Univ Coimbra, Fac Pharm, Grp Pharmaceut Technol, REQUIMTE LAQV, Coimbra - Portugal
Total Affiliations: 6
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 171, p. 501-505, NOV 1 2018.
Web of Science Citations: 7
Abstract

The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations. (AU)

FAPESP's process: 13/21500-1 - Solid lipid nanoparticles for incorporation of trans-resveratrol in the topical treatment of melanoma: in vitro and in vivo evaluation and the elucidation of the mechanism of uptake cellular
Grantee:Roberta Balansin Rigon
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/19568-4 - Evaluation of the potential of nanostructured lipid systems for cutaneous administration of trans-resveratrol
Grantee:Marlus Chorilli
Support type: Regular Research Grants
FAPESP's process: 11/16888-5 - Development and characterization of solid lipid nanoparticles for dermal administration of trans-resveratrol
Grantee:Roberta Balansin Rigon
Support type: Scholarships in Brazil - Master